Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 118141..118784 Replicon plasmid pKPN-10f7
Accession NZ_CP026397
Organism Klebsiella pneumoniae strain KPNIH48

Toxin (Protein)


Gene name vagD Uniprot ID A0A3Q8D6L7
Locus tag C3F39_RS30860 Protein ID WP_020804312.1
Coordinates 118368..118784 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag C3F39_RS30855 Protein ID WP_001261276.1
Coordinates 118141..118371 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3F39_RS30825 113938..114387 - 450 WP_073553761.1 hypothetical protein -
C3F39_RS30830 114459..115169 - 711 WP_073553760.1 hypothetical protein -
C3F39_RS30835 115592..116374 - 783 WP_048268853.1 site-specific integrase -
C3F39_RS30840 116371..117192 - 822 WP_073553765.1 hypothetical protein -
C3F39_RS30845 117233..117562 - 330 WP_048268852.1 hypothetical protein -
C3F39_RS30855 118141..118371 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C3F39_RS30860 118368..118784 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin Toxin
C3F39_RS30865 118825..119703 - 879 WP_032427228.1 restriction endonuclease -
C3F39_RS30870 120365..121642 + 1278 WP_009309915.1 HlyD family secretion protein -
C3F39_RS30875 121632..123740 + 2109 WP_032427226.1 peptidase domain-containing ABC transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..220406 220406


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15154.59 Da        Isoelectric Point: 7.8637

>T94791 WP_020804312.1 NZ_CP026397:118368-118784 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T94791 NZ_CP026397:118368-118784 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT94791 WP_001261276.1 NZ_CP026397:118141-118371 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT94791 NZ_CP026397:118141-118371 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATTCGGCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Q8D6L7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References