Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 63280..63806 Replicon plasmid pKPN-10f7
Accession NZ_CP026397
Organism Klebsiella pneumoniae strain KPNIH48

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag C3F39_RS30500 Protein ID WP_000323025.1
Coordinates 63280..63567 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID J5W3H0
Locus tag C3F39_RS30505 Protein ID WP_004196370.1
Coordinates 63567..63806 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3F39_RS30455 58611..58835 - 225 WP_014343499.1 hypothetical protein -
C3F39_RS30460 58846..59058 - 213 WP_019706020.1 hypothetical protein -
C3F39_RS30465 59119..59475 - 357 WP_019706019.1 hypothetical protein -
C3F39_RS30475 60316..60633 - 318 WP_015065519.1 hypothetical protein -
C3F39_RS30480 60648..60998 - 351 WP_032425551.1 hypothetical protein -
C3F39_RS30485 60995..61267 - 273 WP_032425552.1 hypothetical protein -
C3F39_RS30490 61965..62123 - 159 WP_014343509.1 type I toxin-antitoxin system Hok family toxin -
C3F39_RS30495 62195..63229 + 1035 Protein_72 IS481 family transposase -
C3F39_RS30500 63280..63567 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
C3F39_RS30505 63567..63806 - 240 WP_004196370.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
C3F39_RS30510 64057..64422 + 366 WP_009651956.1 hypothetical protein -
C3F39_RS30515 64466..65203 + 738 WP_008460272.1 HupE/UreJ family protein -
C3F39_RS30520 65217..65906 + 690 WP_004196322.1 hypothetical protein -
C3F39_RS30525 65937..67313 - 1377 Protein_78 chromate efflux transporter -
C3F39_RS30530 67270..68247 - 978 WP_004196334.1 chromate resistance protein -
C3F39_RS30535 68277..68469 + 193 Protein_80 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..220406 220406


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T94790 WP_000323025.1 NZ_CP026397:c63567-63280 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T94790 NZ_CP026397:c63567-63280 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9018.43 Da        Isoelectric Point: 4.3894

>AT94790 WP_004196370.1 NZ_CP026397:c63806-63567 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Download         Length: 240 bp

>AT94790 NZ_CP026397:c63806-63567 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4QRH4

References