Detailed information of TA system TA094789

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4685..5378
• Replicon: plasmid pKPN-10f7
• Accession: NZ_CP026397
• Organism: Klebsiella pneumoniae strain KPNIH48

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: C3F39_RS30140
• Protein ID: WP_000182276.1
• Coordinates: 5022..5378 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: C3F39_RS30135
• Protein ID: WP_001172026.1
• Coordinates: 4685..5020 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3F39_RS30110	357..2063	+	1707	Protein_1	DEAD/DEAH box helicase	-
C3F39_RS30115	2231..3490	-	1260	WP_104457654.1	HD-GYP domain-containing protein	-
C3F39_RS30120	3608..3988	-	381	WP_001054412.1	hypothetical protein	-
C3F39_RS30125	3985..4311	-	327	WP_000091614.1	hypothetical protein	-
C3F39_RS30130	4335..4670	-	336	WP_000741275.1	hypothetical protein	-
C3F39_RS30135	4685..5020	-	336	WP_001172026.1	helix-turn-helix domain-containing protein	Antitoxin
C3F39_RS30140	5022..5378	-	357	WP_000182276.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3F39_RS30145	5570..6172	+	603	WP_010465829.1	recombinase family protein	-
C3F39_RS30150	6156..9185	+	3030	WP_032432545.1	Tn3 family transposase	-
C3F39_RS30155	9154..9711	+	558	WP_104457660.1	ATP-dependent helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..220406	220406

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12912.93 Da
• Isoelectric Point: 8.9018

>T94789 WP_000182276.1 NZ_CP026397:c5378-5022 [Klebsiella pneumoniae]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKELKGFGGAGVLKVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQEL

Nucleotide

• Length: 357 bp

>T94789 NZ_CP026397:c5378-5022 [Klebsiella pneumoniae]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGAGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGAAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT94789 WP_001172026.1 NZ_CP026397:c5020-4685 [Klebsiella pneumoniae]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT94789 NZ_CP026397:c5020-4685 [Klebsiella pneumoniae]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A024EKI7