Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 134994..135519 Replicon plasmid pKPN-8c6e
Accession NZ_CP026396
Organism Klebsiella pneumoniae strain KPNIH48

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag C3F39_RS30060 Protein ID WP_013023785.1
Coordinates 134994..135299 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag C3F39_RS30065 Protein ID WP_006788213.1
Coordinates 135301..135519 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3F39_RS30030 130713..131339 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
C3F39_RS34295 131336..131638 + 303 WP_071571079.1 hypothetical protein -
C3F39_RS30040 132070..132864 - 795 WP_004197635.1 site-specific integrase -
C3F39_RS30045 133062..134078 - 1017 WP_017899884.1 hypothetical protein -
C3F39_RS30050 134089..134403 - 315 WP_053389906.1 hypothetical protein -
C3F39_RS30055 134430..134825 - 396 WP_017899885.1 hypothetical protein -
C3F39_RS30060 134994..135299 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
C3F39_RS30065 135301..135519 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C3F39_RS30080 136117..136698 + 582 WP_101883326.1 hypothetical protein -
C3F39_RS30085 136840..137886 - 1047 WP_104457658.1 IS481-like element ISKpn28 family transposase -
C3F39_RS30090 137984..138916 + 933 WP_101883327.1 Abi family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..144072 144072
- flank IS/Tn - - 136840..137886 1046


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T94788 WP_013023785.1 NZ_CP026396:c135299-134994 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T94788 NZ_CP026396:c135299-134994 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT94788 WP_006788213.1 NZ_CP026396:c135519-135301 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT94788 NZ_CP026396:c135519-135301 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References