Detailed information of TA system TA094787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 41081..41774
• Replicon: plasmid pKPN-8c6e
• Accession: NZ_CP026396
• Organism: Klebsiella pneumoniae strain KPNIH48

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: C3F39_RS29460
• Protein ID: WP_000182276.1
• Coordinates: 41081..41437 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: C3F39_RS29465
• Protein ID: WP_001172026.1
• Coordinates: 41439..41774 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3F39_RS29440	36083..36313	-	231	WP_101883343.1	DUF4113 domain-containing protein	-
C3F39_RS29445	36565..37179	-	615	WP_087859140.1	DUF2913 family protein	-
C3F39_RS29450	37274..40303	-	3030	WP_032432545.1	Tn3 family transposase	-
C3F39_RS29455	40287..40889	-	603	WP_010465829.1	recombinase family protein	-
C3F39_RS29460	41081..41437	+	357	WP_000182276.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3F39_RS29465	41439..41774	+	336	WP_001172026.1	helix-turn-helix domain-containing protein	Antitoxin
C3F39_RS29470	41789..42124	+	336	WP_000741275.1	hypothetical protein	-
C3F39_RS29475	42148..42474	+	327	WP_000091614.1	hypothetical protein	-
C3F39_RS29480	42471..42851	+	381	WP_001054412.1	hypothetical protein	-
C3F39_RS29485	42969..44228	+	1260	WP_104457654.1	HD-GYP domain-containing protein	-
C3F39_RS29490	44813..45808	+	996	WP_020326536.1	IS110 family transposase	-
C3F39_RS29495	45944..46273	-	330	WP_104457655.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..144072	144072

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12912.93 Da
• Isoelectric Point: 8.9018

>T94787 WP_000182276.1 NZ_CP026396:41081-41437 [Klebsiella pneumoniae]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKELKGFGGAGVLKVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQEL

Nucleotide

• Length: 357 bp

>T94787 NZ_CP026396:41081-41437 [Klebsiella pneumoniae]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGAGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGAAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT94787 WP_001172026.1 NZ_CP026396:41439-41774 [Klebsiella pneumoniae]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT94787 NZ_CP026396:41439-41774 [Klebsiella pneumoniae]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A024EKI7