Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1642366..1642985 Replicon chromosome
Accession NZ_CP026392
Organism Klebsiella pneumoniae strain KPNIH48

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag C3F39_RS08630 Protein ID WP_002892050.1
Coordinates 1642767..1642985 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag C3F39_RS08625 Protein ID WP_002892066.1
Coordinates 1642366..1642740 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3F39_RS08615 1637518..1638711 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C3F39_RS08620 1638734..1641880 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
C3F39_RS08625 1642366..1642740 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
C3F39_RS08630 1642767..1642985 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
C3F39_RS08635 1643144..1643710 + 567 WP_002892030.1 maltose O-acetyltransferase -
C3F39_RS33175 1643682..1643822 - 141 WP_004147370.1 hypothetical protein -
C3F39_RS08640 1643843..1644313 + 471 WP_002892026.1 YlaC family protein -
C3F39_RS08645 1644288..1645739 - 1452 WP_040236504.1 PLP-dependent aminotransferase family protein -
C3F39_RS08650 1645840..1646538 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
C3F39_RS08655 1646535..1646675 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
C3F39_RS08660 1646675..1646938 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T94771 WP_002892050.1 NZ_CP026392:1642767-1642985 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T94771 NZ_CP026392:1642767-1642985 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT94771 WP_002892066.1 NZ_CP026392:1642366-1642740 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT94771 NZ_CP026392:1642366-1642740 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References