Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 579635..580328 Replicon chromosome
Accession NZ_CP026386
Organism Pseudomonas sp. PONIH3

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag C3F42_RS02670 Protein ID WP_003151133.1
Coordinates 579635..580012 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag C3F42_RS02675 Protein ID WP_001172026.1
Coordinates 579993..580328 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3F42_RS02655 574746..575591 - 846 WP_003464995.1 AraC family transcriptional regulator -
C3F42_RS02660 575828..578857 - 3030 WP_024015041.1 Tn3 family transposase -
C3F42_RS02665 578841..579443 - 603 WP_010465829.1 recombinase family protein -
C3F42_RS02670 579635..580012 + 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C3F42_RS02675 579993..580328 + 336 WP_001172026.1 helix-turn-helix domain-containing protein Antitoxin
C3F42_RS02680 580343..580677 + 335 Protein_533 transposase -
C3F42_RS02685 580701..581027 + 327 WP_000091614.1 hypothetical protein -
C3F42_RS02690 581024..581404 + 381 WP_001054412.1 hypothetical protein -
C3F42_RS02695 581522..581854 + 333 WP_104443925.1 DUF3391 domain-containing protein -
C3F42_RS02700 581893..582807 + 915 WP_001736514.1 HD domain-containing protein -
C3F42_RS02705 582977..584347 - 1371 Protein_538 diguanylate cyclase -
C3F42_RS02710 584657..584938 + 282 WP_094012136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - entE / basF / basG 403483..670892 267409


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T94764 WP_003151133.1 NZ_CP026386:579635-580012 [Pseudomonas sp. PONIH3]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

>T94764 NZ_CP026386:579635-580012 [Pseudomonas sp. PONIH3]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT94764 WP_001172026.1 NZ_CP026386:579993-580328 [Pseudomonas sp. PONIH3]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp

>AT94764 NZ_CP026386:579993-580328 [Pseudomonas sp. PONIH3]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References