Detailed information of TA system TA094735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1248507..1249135
• Replicon: chromosome
• Accession: NZ_CP026378
• Organism: Mixta calida strain DSM 22759

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A2K1QC31
• Locus tag: C2E16_RS05825
• Protein ID: WP_071883698.1
• Coordinates: 1248507..1248725 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: C2E16_RS05830
• Protein ID: WP_038630095.1
• Coordinates: 1248755..1249135 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2E16_RS05795	1244457..1244795	+	339	WP_038627577.1	P-II family nitrogen regulator	-
C2E16_RS05800	1244832..1246115	+	1284	WP_084970012.1	ammonium transporter AmtB	-
C2E16_RS05805	1246186..1247046	-	861	WP_038627572.1	acyl-CoA thioesterase II	-
C2E16_RS05810	1247261..1247821	+	561	WP_038627570.1	YbaY family lipoprotein	-
C2E16_RS05815	1247856..1248164	-	309	WP_038627568.1	MGMT family protein	-
C2E16_RS05825	1248507..1248725	-	219	WP_071883698.1	hemolysin expression modulator Hha	Toxin
C2E16_RS05830	1248755..1249135	-	381	WP_038630095.1	Hha toxicity modulator TomB	Antitoxin
C2E16_RS05835	1249283..1249636	-	354	WP_038627564.1	hypothetical protein	-
C2E16_RS05840	1249982..1250122	-	141	WP_038627562.1	type B 50S ribosomal protein L36	-
C2E16_RS05845	1250132..1250389	-	258	WP_038627560.1	type B 50S ribosomal protein L31	-
C2E16_RS05850	1250615..1253764	-	3150	WP_084970011.1	multidrug efflux RND transporter permease subunit AcrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8701.16 Da
• Isoelectric Point: 8.8578

>T94735 WP_071883698.1 NZ_CP026378:c1248725-1248507 [Mixta calida]
MTEKIMTKTDYLMRLRRCRSIDTLERVIEKNKYELPDDELAVFYSAADHRLAELTMNKLYDKVPVSVWKYVR

Nucleotide

• Length: 219 bp

>T94735 NZ_CP026378:c1248725-1248507 [Mixta calida]
ATGACAGAAAAAATTATGACCAAGACTGACTATCTGATGCGCTTAAGACGCTGCCGATCAATCGATACGCTTGAGCGAGT
CATTGAAAAAAATAAATATGAATTGCCTGATGATGAGTTGGCTGTGTTTTATTCTGCGGCCGATCATCGCCTTGCGGAAT
TAACGATGAATAAACTGTACGATAAAGTACCCGTTTCGGTGTGGAAATACGTTCGTTGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14755.38 Da
• Isoelectric Point: 4.3024

>AT94735 WP_038630095.1 NZ_CP026378:c1249135-1248755 [Mixta calida]
MDEYSPKRHDIAQLNFLCENLYDESMATLGDSHHGWVNDPTSATNIQLNELIEHIASFTMNYKIKHAEDEDLIVQIDDYL
DDTFMLFSNYGINVQDLQRWQRSARRLFNLFAEECAQLPLQPSHSF

Nucleotide

• Length: 381 bp

>AT94735 NZ_CP026378:c1249135-1248755 [Mixta calida]
ATGGACGAATATTCGCCGAAACGGCATGATATTGCTCAGCTCAATTTCTTGTGTGAAAATCTATACGACGAAAGTATGGC
CACACTGGGAGACAGCCACCATGGCTGGGTCAACGATCCTACATCTGCGACCAACATACAGCTGAATGAGCTGATTGAAC
ATATTGCTTCGTTTACCATGAATTACAAAATAAAACATGCTGAAGATGAGGATCTCATCGTTCAGATTGATGACTACCTC
GACGATACCTTTATGTTATTCAGTAATTACGGGATTAATGTTCAGGATCTACAACGTTGGCAGCGTTCCGCCCGACGTTT
GTTTAATCTTTTCGCAGAAGAGTGCGCTCAGCTCCCTTTACAGCCAAGCCATTCGTTTTAA

Structures

Toxin

• AlphaFold DB: A0A2K1QC31