Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 27720..28245 Replicon plasmid pA708-3
Accession NZ_CP026371
Organism Klebsiella quasipneumoniae strain A708

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag C2767_RS29470 Protein ID WP_001568026.1
Coordinates 27940..28245 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag C2767_RS29465 Protein ID WP_001568025.1
Coordinates 27720..27938 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2767_RS29415 23576..24352 + 777 WP_060617172.1 DUF2971 domain-containing protein -
C2767_RS29420 24488..24742 - 255 WP_009653916.1 hypothetical protein -
C2767_RS29425 24818..25075 - 258 WP_004098928.1 hypothetical protein -
C2767_RS29430 25124..25327 - 204 WP_004098931.1 hemolysin expression modulator Hha -
C2767_RS29435 25361..25729 - 369 WP_101740016.1 hypothetical protein -
C2767_RS29440 25773..26267 - 495 WP_049106297.1 hypothetical protein -
C2767_RS29445 26298..26864 - 567 WP_151114795.1 hypothetical protein -
C2767_RS29450 26861..27124 - 264 WP_013023778.1 hypothetical protein -
C2767_RS29465 27720..27938 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C2767_RS29470 27940..28245 + 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
C2767_RS29475 28414..28809 + 396 WP_017899885.1 hypothetical protein -
C2767_RS29480 28836..29159 + 324 WP_022644730.1 hypothetical protein -
C2767_RS29485 29156..30172 + 1017 WP_020315256.1 hypothetical protein -
C2767_RS29490 30370..31164 + 795 WP_004197635.1 site-specific integrase -
C2767_RS30590 31251..31448 - 198 WP_158236951.1 hypothetical protein -
C2767_RS29495 31604..31783 - 180 WP_006788217.1 hypothetical protein -
C2767_RS29500 31903..32529 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..133085 133085


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T94732 WP_001568026.1 NZ_CP026371:27940-28245 [Klebsiella quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T94732 NZ_CP026371:27940-28245 [Klebsiella quasipneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT94732 WP_001568025.1 NZ_CP026371:27720-27938 [Klebsiella quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT94732 NZ_CP026371:27720-27938 [Klebsiella quasipneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References