Detailed information of TA system TA094731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 71279..72905
• Replicon: plasmid pA708-2
• Accession: NZ_CP026370
• Organism: Klebsiella quasipneumoniae strain A708

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: C2767_RS28970
• Protein ID: WP_103214914.1
• Coordinates: 71580..72905 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: C2767_RS28965
• Protein ID: WP_103214936.1
• Coordinates: 71279..71575 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2767_RS28935	66813..67571	-	759	WP_103214920.1	Tat pathway signal sequence	-
C2767_RS28940	67625..68545	-	921	WP_103214919.1	hypothetical protein	-
C2767_RS28945	68609..68980	-	372	WP_103214918.1	hypothetical protein	-
C2767_RS28950	69121..69810	-	690	WP_103214917.1	transmembrane anchor protein	-
C2767_RS28955	69824..70561	-	738	WP_103214916.1	HupE/UreJ family protein	-
C2767_RS28960	70605..70970	-	366	WP_103214915.1	hypothetical protein	-
C2767_RS28965	71279..71575	+	297	WP_103214936.1	helix-turn-helix transcriptional regulator	Antitoxin
C2767_RS28970	71580..72905	+	1326	WP_103214914.1	type II toxin-antitoxin system HipA family toxin	Toxin
C2767_RS28975	73271..73645	-	375	WP_001568060.1	hypothetical protein	-
C2767_RS28980	73701..74027	-	327	WP_001568059.1	hypothetical protein	-
C2767_RS28985	74024..74752	-	729	WP_001568058.1	plasmid SOS inhibition protein A	-
C2767_RS28990	74749..75180	-	432	WP_020804497.1	conjugation system SOS inhibitor PsiB	-
C2767_RS28995	75225..77282	-	2058	WP_009310025.1	ParB/RepB/Spo0J family partition protein	-
C2767_RS29000	77352..77600	-	249	WP_009310026.1	DUF905 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkA / mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..118719	118719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-68)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50417.59 Da
• Isoelectric Point: 6.2389

>T94731 WP_103214914.1 NZ_CP026370:71580-72905 [Klebsiella quasipneumoniae]
MVRKQQRLAIWMNGIQVGFWEKSKGDDSLRYLPEWVADEQGRPLSLSLPFTPGNQILHGNVVRDYFDNLLPDSEGIRRRL
AMRYQADSLEPFDLLKELGKDCVGAIQLLSDEEEPTDLYSVKYRPLSESEIASTLRNTTEALLPGRHVDNDDLRLSIAGA
QEKTALLWHDGSWCLPLGHTPTTHIFKLPLGLVGNMKADMSTSVENEWLCSLLLEQYGVPVAKTRIAQFEDQKALVVERF
DRRWSGDQQWIIRLPQEDMCQALAVSPLRKYQADGGPGISDIMGVLSHSDRAEHDRIQFFRAQILFWLMAATDGHAKNFS
IAIEPRGQYHLTPLYDVLSAWPIIGNGNAQISWQKCKLAMAIRGSSNYYQIYRILRRHWIKHGEMTGLSRQQIEMMIDDI
VTVTPEVIERVYAQLPAHFPQELAESVFDGMRQQCRRLSEK

Nucleotide

• Length: 1326 bp

>T94731 NZ_CP026370:71580-72905 [Klebsiella quasipneumoniae]
ATGGTCAGGAAACAGCAACGTTTAGCGATATGGATGAACGGCATTCAGGTCGGGTTCTGGGAAAAGAGTAAAGGCGATGA
TTCGTTACGCTATCTCCCTGAATGGGTTGCTGACGAACAAGGTCGGCCATTGTCTCTGTCCCTGCCTTTCACACCCGGCA
ACCAAATTTTGCACGGTAACGTAGTACGTGATTATTTTGATAATTTACTGCCGGATAGTGAAGGGATCCGCAGGCGTCTG
GCAATGCGCTATCAGGCAGACAGTCTCGAACCTTTCGACTTGTTGAAAGAGTTGGGAAAAGACTGCGTGGGGGCAATACA
GCTACTGAGTGATGAAGAGGAACCCACTGATTTATATTCAGTAAAATATCGTCCTCTTTCTGAATCAGAAATCGCGTCAA
CATTACGAAATACGACGGAAGCGTTACTACCCGGCAGGCATGTCGATAATGATGACTTACGATTATCGATTGCCGGTGCG
CAGGAAAAAACAGCTTTACTGTGGCATGACGGCAGTTGGTGTCTGCCTCTGGGTCATACACCGACGACTCATATTTTCAA
GTTACCGCTCGGGCTGGTCGGGAATATGAAAGCAGATATGAGTACGTCAGTAGAAAATGAATGGCTCTGTTCGCTGCTTC
TTGAGCAATACGGGGTCCCTGTCGCCAAAACCCGGATTGCTCAATTTGAAGATCAGAAGGCTCTGGTGGTGGAACGTTTT
GATCGAAGATGGTCGGGAGACCAGCAGTGGATCATTCGCTTACCCCAGGAGGATATGTGCCAGGCTCTCGCGGTATCACC
ATTACGGAAATACCAGGCCGATGGCGGACCAGGGATCTCTGACATTATGGGTGTTCTGAGTCATTCAGACCGGGCTGAAC
ATGACAGAATACAGTTCTTCAGGGCTCAGATTCTTTTCTGGCTTATGGCCGCTACAGATGGCCACGCCAAAAATTTCAGT
ATCGCGATAGAGCCTCGCGGGCAGTATCATCTTACTCCGCTCTATGATGTTTTATCGGCATGGCCAATTATTGGCAACGG
AAATGCACAGATTTCCTGGCAGAAATGCAAACTGGCGATGGCAATCCGTGGCAGCAGTAACTATTACCAGATTTACAGAA
TATTAAGACGCCACTGGATCAAACATGGTGAGATGACTGGTCTTAGCAGGCAGCAGATCGAGATGATGATTGATGACATT
GTAACCGTGACGCCAGAGGTTATTGAACGCGTCTATGCACAACTTCCAGCTCACTTCCCACAAGAACTTGCTGAGAGTGT
TTTTGATGGAATGAGGCAGCAGTGCAGGCGTCTGAGCGAGAAATAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10924.64 Da
• Isoelectric Point: 10.4711

>AT94731 WP_103214936.1 NZ_CP026370:71279-71575 [Klebsiella quasipneumoniae]
MKNVFPIKTLNQLRPLLLGFRKANGLTQKDVSERLGVTQQTYARLEANPASASIERLFKVFSVLGVEISLSSVKGSSEMM
LSGENESLESLPARREKW

Nucleotide

• Length: 297 bp

>AT94731 NZ_CP026370:71279-71575 [Klebsiella quasipneumoniae]
ATGAAAAATGTTTTTCCAATAAAAACACTCAACCAGTTGCGTCCATTGCTGCTTGGATTTCGCAAAGCGAATGGACTGAC
TCAAAAAGACGTTTCTGAGCGGTTAGGCGTAACCCAGCAGACTTACGCTCGCCTGGAGGCCAATCCAGCCAGTGCGAGTA
TTGAACGCCTGTTCAAAGTGTTTTCCGTGCTGGGAGTGGAAATCAGCCTTTCCTCGGTGAAAGGTTCATCAGAAATGATG
CTGTCGGGCGAAAATGAATCTTTGGAAAGTTTGCCCGCCAGACGAGAGAAATGGTGA