Detailed information of TA system TA094730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 27405..28141
• Replicon: plasmid pA708-2
• Accession: NZ_CP026370
• Organism: Klebsiella quasipneumoniae strain A708

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: C2767_RS28645
• Protein ID: WP_004187044.1
• Coordinates: 27405..27887 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: C2767_RS28650
• Protein ID: WP_003026799.1
• Coordinates: 27875..28141 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2767_RS28625	22932..23327	-	396	WP_021312679.1	helix-turn-helix domain-containing protein	-
C2767_RS28630	23376..24722	-	1347	WP_077259381.1	ISNCY family transposase	-
C2767_RS28635	24984..25691	-	708	WP_087796141.1	toll/interleukin-1 receptor domain-containing protein	-
C2767_RS28640	25848..27194	-	1347	WP_151114741.1	ISNCY family transposase	-
C2767_RS28645	27405..27887	-	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
C2767_RS28650	27875..28141	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
C2767_RS28660	29253..30062	-	810	WP_023329017.1	N-formylglutamate deformylase	-
C2767_RS28665	30055..31263	-	1209	WP_057072740.1	imidazolonepropionase	-
C2767_RS28670	31275..31796	-	522	Protein_30	cytosine permease	-
C2767_RS28675	31853..32821	-	969	WP_013815099.1	IS5-like element IS903B family transposase	-
C2767_RS28680	32929..33132	-	204	WP_004150739.1	hemolysin expression modulator Hha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkA / mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..118719	118719
	-	inside	IScluster/Tn	-	-	11333..32821	21488

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T94730 WP_004187044.1 NZ_CP026370:c27887-27405 [Klebsiella quasipneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T94730 NZ_CP026370:c27887-27405 [Klebsiella quasipneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGACGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTTATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT94730 WP_003026799.1 NZ_CP026370:c28141-27875 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT94730 NZ_CP026370:c28141-27875 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66