Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 5329877..5330530 Replicon chromosome
Accession NZ_CP026363
Organism Brevibacillus agri strain DSM 6348

Toxin (Protein)


Gene name mazF Uniprot ID J2PLP3
Locus tag BA6348_RS26210 Protein ID WP_005830205.1
Coordinates 5329877..5330227 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3M8AYL7
Locus tag BA6348_RS26215 Protein ID WP_025845698.1
Coordinates 5330231..5330530 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BA6348_RS26175 5325015..5325812 - 798 WP_005830213.1 RNA polymerase sigma factor SigB -
BA6348_RS26180 5325781..5326275 - 495 WP_005830211.1 anti-sigma B factor RsbW -
BA6348_RS26185 5326275..5326601 - 327 WP_005830210.1 anti-sigma factor antagonist -
BA6348_RS26190 5326627..5327628 - 1002 WP_007776949.1 PP2C family protein-serine/threonine phosphatase -
BA6348_RS26195 5327622..5328059 - 438 WP_026558265.1 anti-sigma regulatory factor -
BA6348_RS26205 5328435..5329748 - 1314 WP_081494728.1 transposase -
BA6348_RS26210 5329877..5330227 - 351 WP_005830205.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BA6348_RS26215 5330231..5330530 - 300 WP_025845698.1 CopG family ribbon-helix-helix protein Antitoxin
BA6348_RS26220 5330717..5331910 - 1194 WP_005830200.1 alanine racemase -
BA6348_RS26225 5331913..5332353 - 441 WP_005830198.1 hypothetical protein -
BA6348_RS26230 5332634..5333755 - 1122 WP_122953518.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5328435..5339206 10771


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-52)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12917.86 Da        Isoelectric Point: 4.8435

>T94716 WP_005830205.1 NZ_CP026363:c5330227-5329877 [Brevibacillus agri]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVNESLQISLGLIDF

Download         Length: 351 bp

>T94716 NZ_CP026363:c5330227-5329877 [Brevibacillus agri]
GTGATTGTCAAACGTGGCGATGTATTTTTCGCGGACCTGTCACCGGTCGTTGGCTCTGAGCAAGGAGGCGTGCGGCCTGT
TTTAGTCATTCAGAACGACATTGGAAATCGATTTAGTCCGACGGTCATCGTCGCCGCCATTACGGCCCAAATTCAGAAGG
CAAAACTGCCTACGCATGTGGAAATTGACGCAAAAACATATGGATTTGATCGTGACTCCGTCATTTTGTTGGAACAAATA
CGAACCATTGACAAACAGAGGCTGACTGATAAGATTACCCATCTTGACGACGAGATGATGGATCGGGTCAATGAGTCTTT
GCAAATCAGCTTGGGACTCATTGATTTCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11323.26 Da        Isoelectric Point: 10.3594

>AT94716 WP_025845698.1 NZ_CP026363:c5330530-5330231 [Brevibacillus agri]
MVGGGFVLSKSNTKRIMISLPQHLLQEVDGVIQKEKSNRSEFIRQAMHLYLKERKKRTIRETMQKGYMEMAKINLKMASE
AFGAEEEADHTLVRLVSGV

Download         Length: 300 bp

>AT94716 NZ_CP026363:c5330530-5330231 [Brevibacillus agri]
ATGGTTGGAGGTGGATTTGTCTTGTCGAAGTCAAACACGAAACGCATCATGATCAGTTTGCCGCAACACTTGCTACAAGA
AGTGGATGGCGTCATTCAAAAGGAGAAATCCAATCGCAGCGAGTTCATTCGCCAGGCGATGCATTTGTATTTGAAGGAGC
GTAAAAAACGTACGATCCGCGAGACAATGCAAAAAGGGTATATGGAGATGGCGAAGATTAATCTGAAAATGGCATCGGAG
GCTTTTGGCGCTGAAGAAGAGGCTGACCATACTCTTGTCCGCTTAGTTAGCGGGGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J2PLP3

Antitoxin

Source ID Structure
AlphaFold DB A0A3M8AYL7

References