Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3011698..3012334 Replicon chromosome
Accession NZ_CP026362
Organism Bacillus vallismortis strain DSM 11031

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BV11031_RS15960 Protein ID WP_003156187.1
Coordinates 3011698..3012048 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag BV11031_RS15965 Protein ID WP_003225183.1
Coordinates 3012053..3012334 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BV11031_RS15920 3006741..3007340 - 600 WP_010331246.1 phosphoserine phosphatase RsbX -
BV11031_RS15925 3007340..3008128 - 789 WP_010331247.1 RNA polymerase sigma factor SigB -
BV11031_RS15930 3008094..3008576 - 483 WP_003234299.1 anti-sigma B factor RsbW -
BV11031_RS15935 3008573..3008902 - 330 WP_010331248.1 anti-sigma factor antagonist RsbV -
BV11031_RS15940 3008964..3009971 - 1008 WP_010331249.1 phosphoserine phosphatase RsbU -
BV11031_RS15945 3009983..3010384 - 402 WP_003240352.1 serine/threonine-protein kinase RsbT -
BV11031_RS15950 3010388..3010753 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
BV11031_RS15955 3010758..3011582 - 825 WP_082022754.1 RsbT co-antagonist protein RsbRA -
BV11031_RS15960 3011698..3012048 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BV11031_RS15965 3012053..3012334 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BV11031_RS15970 3012452..3013621 - 1170 WP_010331251.1 alanine racemase -
BV11031_RS15975 3013736..3014752 - 1017 WP_082022756.1 outer membrane lipoprotein carrier protein LolA -
BV11031_RS15980 3014916..3015281 - 366 WP_010331253.1 holo-ACP synthase -
BV11031_RS15985 3015376..3015975 + 600 WP_010331254.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T94714 WP_003156187.1 NZ_CP026362:c3012048-3011698 [Bacillus vallismortis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T94714 NZ_CP026362:c3012048-3011698 [Bacillus vallismortis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT94714 WP_003225183.1 NZ_CP026362:c3012334-3012053 [Bacillus vallismortis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT94714 NZ_CP026362:c3012334-3012053 [Bacillus vallismortis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References