Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3040425..3041079 Replicon chromosome
Accession NZ_CP026361
Organism Escherichia coli strain 1A

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C2Q99_RS15765 Protein ID WP_000244777.1
Coordinates 3040425..3040832 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C2Q99_RS15770 Protein ID WP_000354046.1
Coordinates 3040813..3041079 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2Q99_RS15745 3036382..3038115 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
C2Q99_RS15750 3038121..3038831 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C2Q99_RS15755 3038856..3039752 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C2Q99_RS15760 3039864..3040385 + 522 WP_001055874.1 flavodoxin FldB -
C2Q99_RS15765 3040425..3040832 - 408 WP_000244777.1 toxin CptA Toxin
C2Q99_RS15770 3040813..3041079 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C2Q99_RS15775 3041322..3042302 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C2Q99_RS15780 3042498..3043157 - 660 WP_000250274.1 hemolysin III family protein -
C2Q99_RS15785 3043321..3043632 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
C2Q99_RS15790 3043677..3045110 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
C2Q99_RS15795 3045167..3045910 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T94692 WP_000244777.1 NZ_CP026361:c3040832-3040425 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T94692 NZ_CP026361:c3040832-3040425 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT94692 WP_000354046.1 NZ_CP026361:c3041079-3040813 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT94692 NZ_CP026361:c3041079-3040813 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References