Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/RHH-PIN |
| Location | 1347608..1348295 | Replicon | chromosome |
| Accession | NC_002754 | ||
| Organism | Sulfolobus solfataricus P2 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | - |
| Locus tag | SSO_RS07285 | Protein ID | WP_080514962.1 |
| Coordinates | 1347608..1347997 (-) | Length | 130 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | - |
| Locus tag | SSO_RS07290 | Protein ID | WP_009991659.1 |
| Coordinates | 1347993..1348295 (-) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SSO_RS07260 (SSO1486) | 1343008..1343346 | - | 339 | WP_010923427.1 | hypothetical protein | - |
| SSO_RS07265 (SSO1487) | 1343471..1344444 | + | 974 | Protein_1435 | IS5 family transposase | - |
| SSO_RS07270 (SSO1489) | 1344586..1345083 | + | 498 | WP_014511660.1 | hypothetical protein | - |
| SSO_RS17025 | 1345479..1345624 | + | 146 | Protein_1437 | IS630 family transposase | - |
| SSO_RS07275 (SSO1490) | 1345806..1346141 | + | 336 | Protein_1438 | transposase | - |
| SSO_RS07280 (SSO1491) | 1346285..1347400 | - | 1116 | WP_009991657.1 | hypothetical protein | - |
| SSO_RS07285 (SSO1493) | 1347608..1347997 | - | 390 | WP_080514962.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| SSO_RS07290 (SSO1494) | 1347993..1348295 | - | 303 | WP_009991659.1 | hypothetical protein | Antitoxin |
| SSO_RS07295 (SSO1495) | 1348773..1349345 | + | 573 | WP_010923431.1 | metallophosphoesterase | - |
| SSO_RS16235 | 1349754..1350421 | - | 668 | Protein_1443 | ISNCY family transposase | - |
| SSO_RS07310 | 1350749..1351292 | + | 544 | Protein_1444 | IS607 family transposase | - |
| SSO_RS07315 (SSO1498) | 1351270..1352527 | + | 1258 | Protein_1445 | transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | - | 1312127..1346141 | 34014 | |
| - | inside | IScluster/Tn | - | - | 1333044..1352472 | 19428 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 130 a.a. Molecular weight: 14458.99 Da Isoelectric Point: 6.4091
>T945 WP_080514962.1 NC_002754:c1347997-1347608 [Saccharolobus solfataricus P2]
IDASVLAKFVTKEKGWEKIREILKDAETLDFALVEVSNVVWKKAVLTGELTGKDVIKAITIVKEYLPQLLTVNKSIDLIE
RAIEISVNEKITVYDSLYIALAECKGSKLVTGDKKQYDVAKKYVISELI
IDASVLAKFVTKEKGWEKIREILKDAETLDFALVEVSNVVWKKAVLTGELTGKDVIKAITIVKEYLPQLLTVNKSIDLIE
RAIEISVNEKITVYDSLYIALAECKGSKLVTGDKKQYDVAKKYVISELI
Download Length: 390 bp
>T945 NC_002754:c1347997-1347608 [Saccharolobus solfataricus P2]
ATTGACGCATCTGTCCTAGCTAAATTTGTAACTAAGGAGAAAGGATGGGAGAAGATTAGAGAGATTTTGAAAGACGCTGA
GACATTAGACTTCGCATTAGTGGAAGTATCTAACGTAGTATGGAAGAAAGCTGTATTAACTGGGGAATTAACTGGGAAAG
ATGTAATTAAGGCAATTACTATTGTTAAAGAGTATTTACCACAACTGCTTACAGTTAACAAAAGCATTGACTTAATTGAA
AGAGCTATTGAGATTTCCGTTAACGAGAAAATCACAGTATATGATTCACTTTATATTGCACTGGCTGAATGTAAGGGGAG
TAAATTGGTAACGGGGGATAAGAAACAATACGACGTAGCTAAAAAGTACGTCATCTCTGAGCTCATATAG
ATTGACGCATCTGTCCTAGCTAAATTTGTAACTAAGGAGAAAGGATGGGAGAAGATTAGAGAGATTTTGAAAGACGCTGA
GACATTAGACTTCGCATTAGTGGAAGTATCTAACGTAGTATGGAAGAAAGCTGTATTAACTGGGGAATTAACTGGGAAAG
ATGTAATTAAGGCAATTACTATTGTTAAAGAGTATTTACCACAACTGCTTACAGTTAACAAAAGCATTGACTTAATTGAA
AGAGCTATTGAGATTTCCGTTAACGAGAAAATCACAGTATATGATTCACTTTATATTGCACTGGCTGAATGTAAGGGGAG
TAAATTGGTAACGGGGGATAAGAAACAATACGACGTAGCTAAAAAGTACGTCATCTCTGAGCTCATATAG
Antitoxin
Download Length: 101 a.a. Molecular weight: 12195.09 Da Isoelectric Point: 4.8275
>AT945 WP_009991659.1 NC_002754:c1348295-1347993 [Saccharolobus solfataricus P2]
MVIKNATILIYVPEIETFIFPVYVYYNMKTVYSLRLDKELREEMEKYNVKWNEEIESFIRKRIEELKKEEILKKINEILQ
TMPETKSNSADLVREDRDNN
MVIKNATILIYVPEIETFIFPVYVYYNMKTVYSLRLDKELREEMEKYNVKWNEEIESFIRKRIEELKKEEILKKINEILQ
TMPETKSNSADLVREDRDNN
Download Length: 303 bp
>AT945 NC_002754:c1348295-1347993 [Saccharolobus solfataricus P2]
ATGGTAATAAAGAATGCTACAATTTTAATCTATGTACCAGAGATAGAAACGTTTATATTTCCCGTATACGTGTATTATAA
TATGAAAACCGTATACAGTTTAAGGTTAGATAAGGAATTACGTGAGGAGATGGAGAAATATAACGTCAAGTGGAATGAGG
AGATAGAGAGCTTTATAAGAAAGAGAATTGAGGAATTGAAGAAAGAGGAGATCTTAAAGAAGATTAATGAAATCCTCCAA
ACAATGCCAGAAACTAAGTCAAATAGTGCAGATTTGGTGAGGGAAGATAGGGATAATAATTGA
ATGGTAATAAAGAATGCTACAATTTTAATCTATGTACCAGAGATAGAAACGTTTATATTTCCCGTATACGTGTATTATAA
TATGAAAACCGTATACAGTTTAAGGTTAGATAAGGAATTACGTGAGGAGATGGAGAAATATAACGTCAAGTGGAATGAGG
AGATAGAGAGCTTTATAAGAAAGAGAATTGAGGAATTGAAGAAAGAGGAGATCTTAAAGAAGATTAATGAAATCCTCCAA
ACAATGCCAGAAACTAAGTCAAATAGTGCAGATTTGGTGAGGGAAGATAGGGATAATAATTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Yukari Maezato et al. (2011) VapC6, a ribonucleolytic toxin regulates thermophilicity in the crenarchaeote Sulfolobus solfataricus. RNA (New York, N.Y.) 17(7):1381-92. [PubMed:21622901]
(2) Charlotte R Cooper et al. (2009) Role of vapBC toxin-antitoxin loci in the thermal stress response of Sulfolobus solfataricus. Biochemical Society Transactions 37(Pt 1):123-6. [PubMed:19143615]
(3) Sabrina Tachdjian et al. (2006) Dynamic metabolic adjustments and genome plasticity are implicated in the heat shock response of the extremely thermoacidophilic archaeon Sulfolobus solfataricus. Journal of Bacteriology 188(12):4553-9. [PubMed:16740961]