Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2077112..2077943 Replicon chromosome
Accession NZ_CP026356
Organism Escherichia coli strain 2FA

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag C2R05_RS11020 Protein ID WP_000854814.1
Coordinates 2077569..2077943 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag C2R05_RS11015 Protein ID WP_001285584.1
Coordinates 2077112..2077480 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2R05_RS11000 2074779..2076311 + 1533 WP_001350525.1 hypothetical protein -
C2R05_RS11005 2076308..2076754 + 447 WP_000187523.1 RadC family protein -
C2R05_RS11010 2076817..2077038 + 222 WP_000692323.1 DUF987 domain-containing protein -
C2R05_RS11015 2077112..2077480 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
C2R05_RS11020 2077569..2077943 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
C2R05_RS11025 2077940..2078134 + 195 WP_000988600.1 hypothetical protein -
C2R05_RS24345 2078147..2078260 + 114 WP_001161660.1 DUF957 domain-containing protein -
C2R05_RS11030 2078549..2078677 - 129 Protein_2014 transposase domain-containing protein -
C2R05_RS11035 2078749..2078931 + 183 WP_001016348.1 hypothetical protein -
C2R05_RS11040 2079032..2079361 - 330 WP_000450409.1 DUF496 family protein -
C2R05_RS11045 2079533..2080591 - 1059 WP_001200891.1 FUSC family protein -
C2R05_RS11050 2080789..2081262 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
C2R05_RS11055 2081381..2082547 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T94472 WP_000854814.1 NZ_CP026356:2077569-2077943 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T94472 NZ_CP026356:2077569-2077943 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT94472 WP_001285584.1 NZ_CP026356:2077112-2077480 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT94472 NZ_CP026356:2077112-2077480 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References