94277

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 263328..264007 Replicon chromosome
Accession NZ_CP026352
Organism Escherichia coli strain 4FA

Toxin (Protein)


Gene name ykfI Uniprot ID P77692
Locus tag C2R11_RS01290 Protein ID WP_000854672.1
Coordinates 263328..263669 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID Q47684
Locus tag C2R11_RS01295 Protein ID WP_000070395.1
Coordinates 263690..264007 (-) Length 106 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2R11_RS01265 258677..259006 + 330 Protein_238 sigma factor-binding protein Crl -
C2R11_RS01270 259045..260100 - 1056 WP_000749863.1 phosphoporin PhoE -
C2R11_RS01275 260388..261491 + 1104 WP_001285288.1 glutamate 5-kinase -
C2R11_RS01280 261503..262756 + 1254 WP_000893278.1 glutamate-5-semialdehyde dehydrogenase -
C2R11_RS01290 263328..263669 - 342 WP_000854672.1 type IV toxin-antitoxin system toxin YkfI Toxin
C2R11_RS01295 263690..264007 - 318 WP_000070395.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
C2R11_RS01300 264026..264247 - 222 WP_000691994.1 DUF987 domain-containing protein -
C2R11_RS01305 264256..264732 - 477 WP_000811693.1 RadC family protein -
C2R11_RS01310 264748..265206 - 459 WP_000211838.1 antirestriction protein -
C2R11_RS01315 265304..265543 - 240 WP_000194654.1 DUF905 domain-containing protein -
C2R11_RS01320 265620..266087 - 468 WP_001547765.1 hypothetical protein -
C2R11_RS01325 266110..266553 - 444 WP_000824223.1 hypothetical protein -
C2R11_RS01330 266553..266780 - 228 WP_001548158.1 hypothetical protein -
C2R11_RS01335 266776..266967 - 192 Protein_251 DeoR family transcriptional regulator -
C2R11_RS01340 267184..268005 - 822 WP_000197389.1 DUF945 domain-containing protein -
C2R11_RS01345 268097..268960 - 864 WP_001065553.1 GTPase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 263108..297021 33913


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(6-104)


Sequences


Toxin        


Download         Length: 114 a.a.        Molecular weight: 12905.01 Da        Isoelectric Point: 9.6543

>T94277 WP_000854672.1 NZ_CP026352:c263669-263328 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T94277 NZ_CP026352:c263669-263328 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11939.59 Da        Isoelectric Point: 6.7391

>AT94277 WP_000070395.1 NZ_CP026352:c264007-263690 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT94277 NZ_CP026352:c264007-263690 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB P77692


Antitoxin

Source ID Structure
AlphaFold DB Q47684

References