Detailed information of TA system TA094012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4533235..4534049
• Replicon: chromosome
• Accession: NZ_CP026345
• Organism: Escherichia coli strain 8A

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: C2R18_RS23420
• Protein ID: WP_001054376.1
• Coordinates: 4533792..4534049 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: C2R18_RS23415
• Protein ID: WP_001309181.1
• Coordinates: 4533235..4533780 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2R18_RS23390	4528926..4530239	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
C2R18_RS23395	4530251..4530529	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
C2R18_RS23400	4530526..4531647	-	1122	WP_000010829.1	M42 family metallopeptidase	-
C2R18_RS24380	4531892..4532008	-	117	Protein_4264	VOC family protein	-
C2R18_RS23405	4532046..4532264	-	219	Protein_4265	hypothetical protein	-
C2R18_RS23410	4532433..4533179	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
C2R18_RS23415	4533235..4533780	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
C2R18_RS23420	4533792..4534049	-	258	WP_001054376.1	hypothetical protein	Toxin
C2R18_RS23430	4534426..4534671	-	246	Protein_4269	GNAT family N-acetyltransferase	-
C2R18_RS23440	4534787..4536027	+	1241	Protein_4270	helicase YjhR	-
C2R18_RS23445	4536610..4537590	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
C2R18_RS23450	4537655..4538761	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimB / fimE	4488557..4542629	54072

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T94012 WP_001054376.1 NZ_CP026345:c4534049-4533792 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T94012 NZ_CP026345:c4534049-4533792 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT94012 WP_001309181.1 NZ_CP026345:c4533780-4533235 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT94012 NZ_CP026345:c4533780-4533235 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8