Detailed information of TA system TA093933

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 263328..264007
• Replicon: chromosome
• Accession: NZ_CP026344
• Organism: Escherichia coli strain 8FA

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: C2R19_RS01290
• Protein ID: WP_000854672.1
• Coordinates: 263328..263669 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: C2R19_RS01295
• Protein ID: WP_000070395.1
• Coordinates: 263690..264007 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2R19_RS01265	258677..259006	+	330	Protein_238	sigma factor-binding protein Crl	-
C2R19_RS01270	259045..260100	-	1056	WP_000749863.1	phosphoporin PhoE	-
C2R19_RS01275	260388..261491	+	1104	WP_001285288.1	glutamate 5-kinase	-
C2R19_RS01280	261503..262756	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
C2R19_RS01290	263328..263669	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
C2R19_RS01295	263690..264007	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
C2R19_RS01300	264026..264247	-	222	WP_000691994.1	DUF987 domain-containing protein	-
C2R19_RS01305	264256..264732	-	477	WP_000811693.1	RadC family protein	-
C2R19_RS01310	264748..265206	-	459	WP_000211838.1	antirestriction protein	-
C2R19_RS01315	265304..265543	-	240	WP_000194654.1	DUF905 domain-containing protein	-
C2R19_RS01320	265620..266087	-	468	WP_001547765.1	hypothetical protein	-
C2R19_RS01325	266110..266553	-	444	WP_000824223.1	hypothetical protein	-
C2R19_RS01330	266553..266780	-	228	WP_001548158.1	hypothetical protein	-
C2R19_RS01335	266776..266967	-	192	Protein_251	DeoR family transcriptional regulator	-
C2R19_RS01340	267184..268005	-	822	WP_000197389.1	DUF945 domain-containing protein	-
C2R19_RS01345	268097..268960	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T93933 WP_000854672.1 NZ_CP026344:c263669-263328 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T93933 NZ_CP026344:c263669-263328 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT93933 WP_000070395.1 NZ_CP026344:c264007-263690 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT93933 NZ_CP026344:c264007-263690 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684