Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 11822..12411 Replicon plasmid pXAG27.EB08
Accession NZ_CP026335
Organism Xanthomonas citri pv. glycines strain EB08

Toxin (Protein)


Gene name pemK Uniprot ID Q32X98
Locus tag C2859_RS24265 Protein ID WP_012477455.1
Coordinates 12079..12411 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID Q32X99
Locus tag C2859_RS24260 Protein ID WP_012477454.1
Coordinates 11822..12079 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2859_RS24230 7547..8467 - 921 WP_012477431.1 RepA -
C2859_RS24235 8533..9339 - 807 WP_016851361.1 serine protease -
C2859_RS24550 9336..9494 - 159 WP_154666355.1 hypothetical protein -
C2859_RS24240 9717..9971 - 255 WP_012477434.1 hypothetical protein -
C2859_RS24245 9971..10621 - 651 WP_012477435.1 ParA family protein -
C2859_RS24250 10688..11332 - 645 WP_012477436.1 recombinase family protein -
C2859_RS24255 11329..11556 - 228 WP_012477437.1 hypothetical protein -
C2859_RS24260 11822..12079 + 258 WP_012477454.1 plasmid stable inheritance protein I Antitoxin
C2859_RS24265 12079..12411 + 333 WP_012477455.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
C2859_RS24270 12500..13318 - 819 WP_012477456.1 hypothetical protein -
C2859_RS24275 13346..15523 - 2178 WP_012477457.1 relaxase/mobilization nuclease domain-containing protein -
C2859_RS24280 15525..15827 - 303 WP_012477458.1 hypothetical protein -
C2859_RS24285 16115..16534 + 420 WP_012477459.1 hypothetical protein -
C2859_RS24290 16524..17228 + 705 WP_007975110.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..26620 26620


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11581.24 Da        Isoelectric Point: 9.1684

>T93841 WP_012477455.1 NZ_CP026335:12079-12411 [Xanthomonas citri pv. glycines]
MERGDIYLVSLDPTSGHEQQGTRPVLIVSPGPFNRLTKTPVVLPITTGGNFARTAGFTVSLTGGGTKTTGVVRCDQPRAL
DIGSRNGRKLESVSAAITDEVMAKLATILE

Download         Length: 333 bp

>T93841 NZ_CP026335:12079-12411 [Xanthomonas citri pv. glycines]
ATGGAGCGGGGTGATATCTATCTTGTGTCTCTCGATCCTACTTCCGGCCATGAGCAACAGGGCACTCGCCCGGTCCTGAT
TGTGTCGCCGGGACCGTTCAACCGCCTTACCAAAACACCCGTGGTGCTACCGATCACCACGGGTGGCAATTTCGCCCGTA
CGGCAGGTTTCACGGTATCCCTCACGGGGGGAGGCACCAAAACGACCGGAGTCGTGCGCTGCGATCAACCGCGCGCCCTG
GACATTGGATCGCGTAACGGCCGCAAGCTGGAAAGCGTCTCAGCAGCGATCACCGATGAGGTGATGGCTAAGCTGGCGAC
CATACTTGAATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 8951.34 Da        Isoelectric Point: 5.5491

>AT93841 WP_012477454.1 NZ_CP026335:11822-12079 [Xanthomonas citri pv. glycines]
MHTTNLRKVGGSVMLAVPPALLDVLHIGAGAKVGLAVDNGRLVVEPHARPRYTLADLLAQCDASAELASEDREWLDAKPV
GGELL

Download         Length: 258 bp

>AT93841 NZ_CP026335:11822-12079 [Xanthomonas citri pv. glycines]
ATGCACACGACAAATCTGCGCAAGGTTGGCGGTTCGGTCATGCTCGCGGTTCCGCCTGCGCTGCTCGATGTGCTGCACAT
CGGGGCAGGGGCAAAAGTGGGCTTGGCTGTCGATAACGGCCGCTTGGTAGTCGAGCCACATGCGCGCCCGCGCTACACCC
TCGCCGACCTGCTCGCCCAATGCGATGCGTCAGCCGAGCTGGCTTCCGAAGATCGGGAATGGCTTGACGCCAAGCCTGTT
GGCGGCGAGCTGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z6Z9Y5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D9EPT3

References