Detailed information of TA system TA093833

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 273727..274595
• Replicon: chromosome
• Accession: NZ_CP026332
• Organism: Pseudomonas sp. XWY-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: PRJ_RS01230
• Protein ID: WP_177429906.1
• Coordinates: 273727..274080 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q88R60
• Locus tag: PRJ_RS01235
• Protein ID: WP_004575922.1
• Coordinates: 274122..274595 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PRJ_RS01215	270017..270736	-	720	WP_060545248.1	response regulator	-
PRJ_RS01220	270877..273174	+	2298	WP_104960611.1	TonB-dependent siderophore receptor	-
PRJ_RS01225	273339..273545	-	207	WP_104962845.1	DUF3079 domain-containing protein	-
PRJ_RS01230	273727..274080	+	354	WP_177429906.1	type II toxin-antitoxin system HigB family toxin	Toxin
PRJ_RS01235	274122..274595	+	474	WP_004575922.1	transcriptional regulator	Antitoxin
PRJ_RS01240	274992..275286	+	295	Protein_228	type II toxin-antitoxin system RelE/ParE family toxin	-
PRJ_RS01245	275416..276171	-	756	WP_034000379.1	IS21-like element ISPre4 family helper ATPase IstB	-
PRJ_RS01250	276192..277706	-	1515	WP_034000381.1	IS21 family transposase	-
PRJ_RS01255	277970..278293	-	324	WP_104960613.1	helix-turn-helix domain-containing protein	-
PRJ_RS01265	278755..279003	-	249	WP_011953231.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (73-111)

Antitoxin

• Antitoxin HigA (43-154)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13700.94 Da
• Isoelectric Point: 10.6182

>T93833 WP_177429906.1 NZ_CP026332:273727-274080 [Pseudomonas sp. XWY-1]
MRVITKAAVTKAIEVHGQWKAPLSLWLTTFDRATLRFESFEQLRQTWATVSGWNVDRIPHSKLRPVSRKGPLDIYVFDIK
KNECRLIAWLNARTGTLYIKAILSHAEYDKWCRSDIR

Nucleotide

• Length: 354 bp

>T93833 NZ_CP026332:273727-274080 [Pseudomonas sp. XWY-1]
ATGCGGGTCATAACGAAAGCGGCGGTTACCAAGGCGATTGAAGTTCACGGCCAGTGGAAAGCGCCCCTAAGCCTGTGGCT
GACGACCTTCGACAGGGCGACGCTGCGGTTTGAATCTTTTGAACAGTTGAGGCAGACGTGGGCCACTGTGTCTGGCTGGA
ATGTTGATCGCATCCCCCACTCAAAACTCAGGCCCGTCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAG
AAAAACGAATGCCGTCTGATCGCGTGGCTCAATGCGCGAACGGGCACTCTCTACATCAAGGCCATTCTGTCGCACGCCGA
GTACGACAAATGGTGCAGAAGCGATATCAGGTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17229.67 Da
• Isoelectric Point: 4.4982

>AT93833 WP_004575922.1 NZ_CP026332:274122-274595 [Pseudomonas sp. XWY-1]
MKRDKIEANGLDAFIANISPMLDGLNTQMATMAQLLAACHDPIKDDAELEARMALIDELYSHADSEGHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLSAIATQSAVSEILNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT93833 NZ_CP026332:274122-274595 [Pseudomonas sp. XWY-1]
ATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCCTGAACAC
TCAGATGGCAACCATGGCCCAGCTTCTAGCCGCCTGCCATGACCCCATCAAGGACGATGCCGAGCTTGAGGCACGCATGG
CCTTGATCGACGAGCTCTACTCCCATGCGGACAGCGAAGGCCACGCTGCGGCACGCTTTGCCGAGATGGTAGCGGACCGC
GTCTACGAGTATGAGAGTGAGTCTGTATTGGTGCCGTTCGCGAGCCAGGCCGAAGCGTTGGCCTTTCTGCTGTCGGACAG
GGGCGTGAAGCAGAAGGACCTGTCGGCGATAGCGACCCAGAGCGCGGTATCCGAGATTCTGAACAACAAGCGGAAGATGA
CGGTTGCCCAGATAAAGGGCTTTGCTGAGTTTTTCAGCGTACCGGTTGAGTTCTTCATGCATGGGGTGGTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2I0CBJ4