Detailed information of TA system TA093832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-parD/RelE-RHH
• Location: 45151..45722
• Replicon: plasmid p1
• Accession: NZ_CP026329
• Organism: Acidithiobacillus caldus strain MTH-04

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F9ZUQ2
• Locus tag: A5904_RS13895
• Protein ID: WP_014003668.1
• Coordinates: 45438..45722 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: F9ZUQ1
• Locus tag: A5904_RS13890
• Protein ID: WP_014003667.1
• Coordinates: 45151..45441 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5904_RS13840	40211..40333	+	123	WP_187288691.1	transposase	-
A5904_RS13845	40479..40943	-	465	WP_014003661.1	SOS-response repressor and protease LexA	-
A5904_RS13850	40967..41272	-	306	WP_148262249.1	hypothetical protein	-
A5904_RS13855	41241..41525	-	285	WP_049784765.1	hypothetical protein	-
A5904_RS13860	41799..42731	+	933	WP_014003663.1	tyrosine-type recombinase/integrase	-
A5904_RS13865	43324..43599	+	276	WP_041638124.1	HU family DNA-binding protein	-
A5904_RS13875	43871..44146	+	276	WP_041638124.1	HU family DNA-binding protein	-
A5904_RS13880	44158..44520	+	363	WP_014003665.1	hypothetical protein	-
A5904_RS13885	44611..44922	+	312	WP_014003666.1	integration host factor subunit alpha	-
A5904_RS13890	45151..45441	+	291	WP_014003667.1	hypothetical protein	Antitoxin
A5904_RS13895	45438..45722	+	285	WP_014003668.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
A5904_RS13900	45999..46595	-	597	WP_103415156.1	uracil-DNA glycosylase	-
A5904_RS13905	46592..46825	-	234	WP_014003670.1	hypothetical protein	-
A5904_RS13910	46840..47537	-	698	Protein_59	prepilin peptidase	-
A5904_RS13915	47613..47945	-	333	WP_014003673.1	hypothetical protein	-
A5904_RS13920	47997..49043	-	1047	WP_103415157.1	IS630 family transposase	-
A5904_RS13925	49094..49354	+	261	Protein_62	HipA N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..190798	190798

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-89)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10643.24 Da
• Isoelectric Point: 6.8795

>T93832 WP_014003668.1 NZ_CP026329:45438-45722 [Acidithiobacillus caldus]
VKLFWTPEAIQDRDDIYAHIEADNPAAALALDELFSEKAARLVDHPGQGRSGRVVGTRELVVHRNYILVYDLAEGMVRVL
RVLHAARQWPPVKM

Nucleotide

• Length: 285 bp

>T93832 NZ_CP026329:45438-45722 [Acidithiobacillus caldus]
GTGAAACTGTTCTGGACACCAGAGGCGATACAAGACCGCGACGACATCTATGCCCATATCGAGGCCGACAATCCGGCCGC
CGCACTGGCTCTCGATGAGCTGTTTTCAGAGAAGGCTGCTCGCCTGGTAGATCACCCAGGTCAGGGCCGGTCTGGGCGCG
TTGTCGGCACCCGCGAGCTGGTAGTGCATAGGAATTACATTCTGGTCTATGACTTGGCAGAGGGCATGGTTCGCGTGTTG
CGTGTCCTGCACGCGGCTCGACAGTGGCCACCAGTGAAGATGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10989.39 Da
• Isoelectric Point: 10.2812

>AT93832 WP_014003667.1 NZ_CP026329:45151-45441 [Acidithiobacillus caldus]
MSEATFTFRVDEALKIEFSKAAKARDRSGAQLLRDFMREFVQQQQEATAHDAWFRRQVQIGMDSANAGNLIPAAEVEARF
AAKRVATRRRLEASEW

Nucleotide

• Length: 291 bp

>AT93832 NZ_CP026329:45151-45441 [Acidithiobacillus caldus]
ATGAGCGAAGCTACCTTTACTTTTCGGGTCGATGAAGCCTTGAAAATCGAGTTCTCCAAGGCCGCTAAGGCGCGCGACCG
CAGCGGTGCGCAACTCCTGCGCGACTTCATGCGTGAGTTCGTGCAGCAGCAACAGGAAGCGACCGCGCATGACGCATGGT
TCCGTCGCCAGGTGCAAATTGGCATGGATTCGGCTAATGCAGGTAACCTGATTCCAGCGGCCGAGGTGGAAGCCAGGTTT
GCGGCCAAGCGGGTGGCCACGCGTCGCCGACTTGAAGCCTCCGAATGGTGA

Structures

Toxin

• AlphaFold DB: F9ZUQ2

Antitoxin

• AlphaFold DB: F9ZUQ1