Detailed information of TA system TA093829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 846572..847167
• Replicon: chromosome
• Accession: NZ_CP026328
• Organism: Acidithiobacillus caldus strain MTH-04

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: F9ZNF3
• Locus tag: A5904_RS04085
• Protein ID: WP_014002921.1
• Coordinates: 846871..847167 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: A5904_RS04080
• Protein ID: WP_004872065.1
• Coordinates: 846572..846865 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5904_RS04060	841944..842363	-	420	WP_038471791.1	hypothetical protein	-
A5904_RS04065	842376..843380	+	1005	WP_188092790.1	GGDEF domain-containing protein	-
A5904_RS04070	843428..845053	+	1626	WP_014002923.1	GGDEF domain-containing protein	-
A5904_RS04075	845377..846336	+	960	WP_103415125.1	GTP-binding protein	-
A5904_RS04080	846572..846865	-	294	WP_004872065.1	putative addiction module antidote protein	Antitoxin
A5904_RS04085	846871..847167	-	297	WP_014002921.1	addiction module antitoxin RelB	Toxin
A5904_RS04100	848340..848549	+	210	WP_103415044.1	hypothetical protein	-
A5904_RS04105	848613..849320	+	708	WP_004872063.1	MgtC/SapB family protein	-
A5904_RS04110	849580..850773	+	1194	WP_004872061.1	divalent metal cation transporter	-
A5904_RS04115	850795..851712	-	918	WP_004872060.1	magnesium transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (6-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11235.09 Da
• Isoelectric Point: 9.9555

>T93829 WP_014002921.1 NZ_CP026328:c847167-846871 [Acidithiobacillus caldus]
MKEIIKSDIFDRWLIALRDRRARARIEVRIRRLSLGNPGDVKPVGEGVLEIRIDYDPGYHVYFMSRGPLVIVLLCAGDKS
SQDRDIALAKNIAAQWKE

Nucleotide

• Length: 297 bp

>T93829 NZ_CP026328:c847167-846871 [Acidithiobacillus caldus]
ATGAAGGAAATCATCAAATCCGATATTTTCGATCGCTGGCTTATCGCACTTCGTGACCGACGAGCCCGAGCAAGGATCGA
GGTGCGAATTCGGCGATTGAGCCTTGGTAATCCCGGAGATGTAAAACCAGTAGGGGAAGGCGTATTGGAAATACGGATTG
ATTACGATCCAGGTTACCACGTCTATTTTATGAGTCGTGGCCCATTGGTCATAGTACTGCTTTGTGCTGGCGACAAAAGC
AGTCAGGACCGGGATATTGCCTTGGCCAAGAACATTGCAGCACAGTGGAAGGAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10471.97 Da
• Isoelectric Point: 5.0634

>AT93829 WP_004872065.1 NZ_CP026328:c846865-846572 [Acidithiobacillus caldus]
MDIQFSRYDTVDYLQTEEDMVAYLDAVMEDGDPALIAAALGDIARARNLSRLAREVGMSRQGLDKALSGKGNPSLATVLK
VAKALGLRLAFQTARQG

Nucleotide

• Length: 294 bp

>AT93829 NZ_CP026328:c846865-846572 [Acidithiobacillus caldus]
ATGGACATCCAATTCAGTCGCTATGACACGGTAGACTACCTTCAGACCGAGGAAGACATGGTGGCTTATCTCGATGCGGT
GATGGAAGACGGCGACCCTGCATTGATTGCCGCAGCGCTGGGGGACATAGCCCGCGCCCGCAATCTCAGCCGCCTCGCCC
GAGAAGTCGGAATGAGCCGCCAGGGGCTGGACAAGGCCCTTTCGGGCAAAGGCAACCCCAGCTTGGCTACGGTCCTCAAG
GTCGCCAAGGCGCTCGGGCTAAGACTGGCCTTTCAAACAGCCAGACAAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A1E7YSJ9