Detailed information of TA system TA093814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 27965..28524
• Replicon: plasmid unnamed 4
• Accession: NZ_CP026320
• Organism: Vibrio campbellii strain BoB-90

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2K7SRU8
• Locus tag: C1N51_RS29470
• Protein ID: WP_017035198.1
• Coordinates: 28246..28524 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A347UR21
• Locus tag: C1N51_RS29465
• Protein ID: WP_005398409.1
• Coordinates: 27965..28249 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1N51_RS29415	23778..24407	-	630	WP_103412365.1	hypothetical protein	-
C1N51_RS29420	24435..24566	-	132	WP_103412366.1	DUF3265 domain-containing protein	-
C1N51_RS29425	24653..24916	+	264	WP_103412367.1	hypothetical protein	-
C1N51_RS29430	25180..25971	-	792	WP_103412368.1	PhzF family phenazine biosynthesis protein	-
C1N51_RS29435	26004..26135	-	132	WP_079855550.1	DUF3265 domain-containing protein	-
C1N51_RS29445	26623..26715	-	93	WP_080765011.1	DUF3265 domain-containing protein	-
C1N51_RS29450	26730..27134	-	405	WP_158652736.1	hypothetical protein	-
C1N51_RS29455	27280..27768	-	489	WP_103412370.1	ATP-binding protein	-
C1N51_RS29930	27797..27888	-	92	Protein_50	DUF3265 domain-containing protein	-
C1N51_RS29465	27965..28249	+	285	WP_005398409.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
C1N51_RS29470	28246..28524	+	279	WP_017035198.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
C1N51_RS29475	28553..28645	-	93	WP_140256639.1	DUF3265 domain-containing protein	-
C1N51_RS29485	29081..30112	+	1032	WP_199770569.1	IS630 family transposase	-
C1N51_RS29490	30260..31789	-	1530	WP_103410819.1	IS66 family transposase	-
C1N51_RS29495	31849..32202	-	354	WP_103410057.1	IS66 family insertion sequence element accessory protein TnpB	-
C1N51_RS29500	32199..32516	-	318	WP_103410056.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37211	37211
	-	inside	Integron	-	-	3..37145	37142

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10905.60 Da
• Isoelectric Point: 4.3430

>T93814 WP_017035198.1 NZ_CP026320:28246-28524 [Vibrio campbellii]
MILWEEESLNDREKIFEFLYDFNPDAAEKTDNLIEAKVENLLEQPLMGVQRDGIRGRLLIIPEISMIVSYWIEGDIIRVM
RVLHQKQKFPTD

Nucleotide

• Length: 279 bp

>T93814 NZ_CP026320:28246-28524 [Vibrio campbellii]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAGATCTTCGAGTTTCTCTATGACTTCAACCCTGATGCTGC
AGAAAAAACTGACAACCTCATTGAAGCAAAAGTAGAAAACTTGCTTGAACAACCTTTAATGGGGGTACAACGAGATGGCA
TCCGCGGACGATTACTCATCATTCCTGAGATTTCGATGATTGTCTCTTACTGGATTGAGGGCGATATCATCCGAGTTATG
CGTGTACTCCACCAGAAACAAAAATTCCCTACGGATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 11069.40 Da
• Isoelectric Point: 9.2167

>AT93814 WP_005398409.1 NZ_CP026320:27965-28249 [Vibrio campbellii]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKTLSHDAWLTEQVNLAFEKFDSGKSVFVEHQTAKSR
MEERKARIRNRGKQ

Nucleotide

• Length: 285 bp

>AT93814 NZ_CP026320:27965-28249 [Vibrio campbellii]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCTGAGAGCCAAGGTCGCAC
TCTGAGTGACGCATGTCGTGAACTTACTGAGCAACTCGCTGAACAACAAAGAAAAACATTATCTCACGATGCATGGCTAA
CTGAACAAGTAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAATCTGTTTTCGTTGAGCACCAAACTGCTAAGTCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGTAATCGAGGTAAGCAATGA

Structures

Toxin

• AlphaFold DB: A0A2K7SRU8

Antitoxin

• AlphaFold DB: A0A347UR21