Detailed information of TA system TA093812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 59696..60282
• Replicon: plasmid unnamed 2
• Accession: NZ_CP026318
• Organism: Vibrio campbellii strain BoB-90

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C1N51_RS28620
• Protein ID: WP_103412262.1
• Coordinates: 59696..60025 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: C1N51_RS28625
• Protein ID: WP_103412263.1
• Coordinates: 60025..60282 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1N51_RS28580	54905..55399	+	495	WP_158652732.1	hypothetical protein	-
C1N51_RS28585	55415..56554	+	1140	WP_103412256.1	tyrosine-type recombinase/integrase	-
C1N51_RS28590	56653..57066	+	414	WP_103412257.1	hypothetical protein	-
C1N51_RS28595	57071..57391	+	321	Protein_63	hypothetical protein	-
C1N51_RS28600	57394..57681	+	288	WP_103412258.1	hypothetical protein	-
C1N51_RS28610	57984..58331	+	348	WP_103412260.1	hypothetical protein	-
C1N51_RS28615	58347..59681	-	1335	WP_103412261.1	replicative DNA helicase	-
C1N51_RS28620	59696..60025	-	330	WP_103412262.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C1N51_RS28625	60025..60282	-	258	WP_103412263.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C1N51_RS28630	60552..61178	+	627	WP_031847632.1	hypothetical protein	-
C1N51_RS28635	61175..61489	+	315	WP_031847633.1	hypothetical protein	-
C1N51_RS28640	61486..62664	+	1179	WP_103412264.1	hypothetical protein	-
C1N51_RS28645	62664..63404	+	741	WP_031847635.1	hypothetical protein	-
C1N51_RS28650	63401..64324	+	924	WP_103412265.1	chemotaxis protein	-
C1N51_RS28655	64327..64524	+	198	WP_103412266.1	hypothetical protein	-
C1N51_RS28660	64554..65234	+	681	WP_103412267.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..74233	74233

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-99)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12102.15 Da
• Isoelectric Point: 9.6897

>T93812 WP_103412262.1 NZ_CP026318:c60025-59696 [Vibrio campbellii]
MYVPDKGDIVSLDFDPSAGKEIMKRRPAFVISRKMFNEHTGFAVVAPITSTVRGMKLEVVLPEDLSTQGAILIHQVKSLD
FSNRQVKFIEKAPQAITDKATELTKVIIS

Nucleotide

• Length: 330 bp

>T93812 NZ_CP026318:c60025-59696 [Vibrio campbellii]
ATGTACGTACCAGATAAAGGAGACATTGTTTCACTCGACTTTGATCCGAGCGCAGGAAAGGAAATCATGAAACGTCGCCC
TGCGTTTGTGATCTCTCGTAAAATGTTCAATGAACACACTGGATTTGCTGTTGTAGCTCCAATCACTAGCACAGTGAGAG
GTATGAAGCTGGAAGTCGTTTTGCCTGAAGATCTATCGACACAAGGCGCCATCCTCATTCACCAAGTTAAATCTCTCGAT
TTCTCTAACCGTCAGGTCAAATTCATAGAGAAAGCCCCACAAGCAATCACCGACAAAGCCACTGAGCTTACAAAAGTGAT
TATTTCGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9270.77 Da
• Isoelectric Point: 4.5822

>AT93812 WP_103412263.1 NZ_CP026318:c60282-60025 [Vibrio campbellii]
MTAVVIRQSGGANIVSIPKTIVKMLSLHAGSKLDLSVQDNRIVLTPVEEELTLEALLAESPKECFAITDEDREWINAKPV
GKEVI

Nucleotide

• Length: 258 bp

>AT93812 NZ_CP026318:c60282-60025 [Vibrio campbellii]
ATGACCGCTGTTGTAATTCGCCAGTCTGGCGGCGCTAACATCGTTAGCATCCCTAAAACCATCGTTAAAATGCTTAGCTT
GCACGCTGGTTCAAAACTGGACCTATCCGTTCAAGATAACAGAATCGTGCTAACTCCGGTTGAGGAAGAGCTAACGTTGG
AAGCCTTACTCGCTGAAAGCCCAAAAGAGTGCTTTGCCATTACCGATGAAGACAGAGAATGGATTAATGCTAAGCCTGTT
GGCAAGGAAGTGATTTGA