Detailed information of TA system TA093804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2222569..2223154
• Replicon: chromosome
• Accession: NZ_CP026307
• Organism: Ralstonia solanacearum strain IBSBF 2571

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: F6G268
• Locus tag: C2124_RS10265
• Protein ID: WP_014617297.1
• Coordinates: 2222569..2222877 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F6G269
• Locus tag: C2124_RS10270
• Protein ID: WP_014617298.1
• Coordinates: 2222870..2223154 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2124_RS10245	2218742..2219659	-	918	WP_174265303.1	sugar ABC transporter substrate-binding protein	-
C2124_RS10250	2219869..2220921	-	1053	WP_039549673.1	LacI family DNA-binding transcriptional regulator	-
C2124_RS10255	2221056..2222213	+	1158	WP_052307006.1	fatty acid desaturase family protein	-
C2124_RS10260	2222179..2222490	+	312	WP_014617296.1	Rieske 2Fe-2S domain-containing protein	-
C2124_RS10265	2222569..2222877	+	309	WP_014617297.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2124_RS10270	2222870..2223154	+	285	WP_014617298.1	putative addiction module antidote protein	Antitoxin
C2124_RS10275	2223168..2224013	-	846	WP_014617299.1	TIM barrel protein	-
C2124_RS10280	2224010..2225047	-	1038	WP_014617300.1	hypothetical protein	-
C2124_RS10285	2225044..2226333	-	1290	WP_174265304.1	FAD-dependent oxidoreductase	-
C2124_RS10290	2226501..2227417	+	917	Protein_2017	MurR/RpiR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (6-91)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11361.99 Da
• Isoelectric Point: 9.8179

>T93804 WP_014617297.1 NZ_CP026307:2222569-2222877 [Ralstonia solanacearum]
MHTLIRSDTFDQWLEKLSDKRVKARITARLVSASFGNVGDCKPVGEGISEMRIDVGAGYRVYYTRQGTVVYILLTGGSKA
TQAQDIKQAIRMARELKESGHD

Nucleotide

• Length: 309 bp

>T93804 NZ_CP026307:2222569-2222877 [Ralstonia solanacearum]
ATGCATACGCTGATCCGCTCCGACACGTTCGACCAGTGGCTTGAAAAGCTGAGCGACAAGCGCGTCAAGGCGCGGATCAC
CGCCCGGCTGGTCAGCGCATCATTCGGGAATGTTGGCGACTGCAAGCCCGTGGGGGAAGGCATCAGCGAAATGCGGATCG
ATGTGGGCGCCGGGTATCGCGTCTACTACACGCGACAAGGTACCGTCGTCTACATCCTGCTCACGGGCGGCAGCAAAGCA
ACCCAAGCCCAGGATATCAAGCAAGCCATCCGGATGGCACGCGAGTTGAAGGAGTCAGGCCATGACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 9905.28 Da
• Isoelectric Point: 4.7361

>AT93804 WP_014617298.1 NZ_CP026307:2222870-2223154 [Ralstonia solanacearum]
MTETYAPFDAAAYLDSEETIAEFLAAAMEDPNPDVFLAALGAVARARGIAKVAADAGLGRESLYKAISPGAHPRYETVQK
IVRALGVRLNVTPA

Nucleotide

• Length: 285 bp

>AT93804 NZ_CP026307:2222870-2223154 [Ralstonia solanacearum]
ATGACTGAGACCTACGCACCGTTTGACGCGGCGGCCTATCTCGATAGCGAAGAGACCATCGCCGAATTTCTGGCCGCCGC
CATGGAAGACCCGAATCCGGACGTCTTCCTGGCTGCGCTCGGCGCCGTCGCGCGCGCCCGCGGCATTGCCAAGGTGGCCG
CGGACGCGGGCCTGGGGCGTGAAAGCCTGTACAAGGCCATCAGCCCGGGCGCACATCCACGCTACGAAACCGTCCAGAAA
ATCGTGCGCGCATTGGGCGTCAGGTTGAACGTGACGCCCGCTTGA

Structures

Toxin

• AlphaFold DB: F6G268

Antitoxin

• AlphaFold DB: F6G269