Detailed information of TA system TA093801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 193220..193863
• Replicon: plasmid pKOR-ab4d
• Accession: NZ_CP026276
• Organism: Klebsiella oxytoca strain KONIH5

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: C2U46_RS31430
• Protein ID: WP_016236302.1
• Coordinates: 193447..193863 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: C2U46_RS31425
• Protein ID: WP_001261282.1
• Coordinates: 193220..193450 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U46_RS31385	188304..188588	+	285	WP_000537152.1	IS3 family transposase	-
C2U46_RS31390	188585..189439	+	855	WP_077253370.1	IS3 family transposase	-
C2U46_RS31395	189538..189855	+	318	WP_103449271.1	hypothetical protein	-
C2U46_RS31400	189936..190718	-	783	WP_032694612.1	site-specific integrase	-
C2U46_RS31405	190916..191920	-	1005	WP_032694611.1	hypothetical protein	-
C2U46_RS31410	192107..192412	-	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	-
C2U46_RS31415	192414..192632	-	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	-
C2U46_RS32680	192802..193263	-	462	Protein_229	hypothetical protein	-
C2U46_RS31425	193220..193450	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C2U46_RS31430	193447..193863	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
C2U46_RS31435	193937..195499	+	1563	WP_004206609.1	AAA family ATPase	-
C2U46_RS31440	195484..196506	+	1023	WP_000361404.1	helicase UvrD	-
C2U46_RS31445	197044..197958	+	915	WP_031592169.1	HNH endonuclease	-
C2U46_RS31450	198144..198494	-	351	WP_004187110.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..282476	282476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T93801 WP_016236302.1 NZ_CP026276:193447-193863 [Klebsiella oxytoca]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T93801 NZ_CP026276:193447-193863 [Klebsiella oxytoca]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT93801 WP_001261282.1 NZ_CP026276:193220-193450 [Klebsiella oxytoca]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT93801 NZ_CP026276:193220-193450 [Klebsiella oxytoca]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3