Detailed information of TA system TA093799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 186610..187361
• Replicon: plasmid pKOR-ab4d
• Accession: NZ_CP026276
• Organism: Klebsiella oxytoca strain KONIH5

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: C2U46_RS31375
• Protein ID: WP_032694614.1
• Coordinates: 186879..187361 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: C2U46_RS31370
• Protein ID: WP_004902250.1
• Coordinates: 186610..186888 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U46_RS31340	181683..182972	-	1290	WP_032694618.1	arsenic transporter	-
C2U46_RS31345	183019..184770	-	1752	WP_032694617.1	arsenical pump-driving ATPase	-
C2U46_RS31350	184787..185149	-	363	WP_032694616.1	arsenite efflux transporter metallochaperone ArsD	-
C2U46_RS31355	185197..185550	-	354	WP_025376942.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
C2U46_RS31360	185854..186195	+	342	WP_032694615.1	hypothetical protein	-
C2U46_RS31365	186279..186473	+	195	Protein_218	hypothetical protein	-
C2U46_RS31370	186610..186888	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
C2U46_RS31375	186879..187361	+	483	WP_032694614.1	GNAT family N-acetyltransferase	Toxin
C2U46_RS31380	187717..188097	-	381	WP_032694621.1	hypothetical protein	-
C2U46_RS31385	188304..188588	+	285	WP_000537152.1	IS3 family transposase	-
C2U46_RS31390	188585..189439	+	855	WP_077253370.1	IS3 family transposase	-
C2U46_RS31395	189538..189855	+	318	WP_103449271.1	hypothetical protein	-
C2U46_RS31400	189936..190718	-	783	WP_032694612.1	site-specific integrase	-
C2U46_RS31405	190916..191920	-	1005	WP_032694611.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..282476	282476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17678.45 Da
• Isoelectric Point: 8.9130

>T93799 WP_032694614.1 NZ_CP026276:186879-187361 [Klebsiella oxytoca]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENSNRVIGYYCLSSGSVHRNTVPGAYRRNAP
EVIPVIVLGRLAIDQAWSGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T93799 NZ_CP026276:186879-187361 [Klebsiella oxytoca]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACTCGAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GAAGTCATTCCGGTCATTGTCCTGGGACGGCTTGCCATCGACCAGGCCTGGTCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATTGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT93799 WP_004902250.1 NZ_CP026276:186610-186888 [Klebsiella oxytoca]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT93799 NZ_CP026276:186610-186888 [Klebsiella oxytoca]
ATGAAACCAGACGTTCGTGAAGCTCCGATCAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAAGCACAGGACATTCTACTTGATCAACGAC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3