Detailed information of TA system TA093798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 162487..163022
• Replicon: plasmid pKOR-ab4d
• Accession: NZ_CP026276
• Organism: Klebsiella oxytoca strain KONIH5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6M4NRI2
• Locus tag: C2U46_RS31245
• Protein ID: WP_032693963.1
• Coordinates: 162735..163022 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C2U46_RS31240
• Protein ID: WP_032693964.1
• Coordinates: 162487..162738 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U46_RS31200	157618..157920	-	303	Protein_188	hypothetical protein	-
C2U46_RS31205	157914..158324	-	411	WP_103449263.1	DUF1380 family protein	-
C2U46_RS31210	158371..158592	-	222	WP_032693969.1	hypothetical protein	-
C2U46_RS31215	158592..159293	-	702	WP_032693968.1	DNA methylase	-
C2U46_RS31225	160181..160411	-	231	WP_032693967.1	hypothetical protein	-
C2U46_RS31230	160621..161046	+	426	WP_032693966.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
C2U46_RS31235	161046..162320	+	1275	WP_032693965.1	Y-family DNA polymerase	-
C2U46_RS31240	162487..162738	+	252	WP_032693964.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C2U46_RS31245	162735..163022	+	288	WP_032693963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2U46_RS32465	163026..163202	+	177	WP_158657247.1	hypothetical protein	-
C2U46_RS31250	163341..163637	-	297	Protein_198	IS3 family transposase	-
C2U46_RS31255	163654..164736	-	1083	WP_103449265.1	IS110 family transposase	-
C2U46_RS31265	165016..165683	-	668	Protein_200	IS3 family transposase	-
C2U46_RS31270	165750..166718	-	969	WP_199770090.1	IS5 family transposase	-
C2U46_RS31275	167293..167928	-	636	WP_071993388.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..282476	282476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11133.12 Da
• Isoelectric Point: 10.2246

>T93798 WP_032693963.1 NZ_CP026276:162735-163022 [Klebsiella oxytoca]
MTYTVKFREDALKEWQKLDKAIQQQFAKKLKKCCDEPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T93798 NZ_CP026276:162735-163022 [Klebsiella oxytoca]
ATGACTTATACGGTCAAATTCAGGGAAGATGCGCTGAAGGAATGGCAAAAGCTGGATAAGGCCATTCAGCAACAGTTTGC
GAAGAAGCTGAAGAAGTGCTGTGACGAACCACATATCCCCTCAGCAAAGCTGAGAGGGATAAAGGACTGCTATAAAATTA
AACTTCGCGCGTCAGGCTTTCGTCTGGTCTATCAGGTAATAGATGATCAACTGATTATTGCTGTTGTGGCTGTCGGAAAG
CGTGAACGTAGTGACGTGTACAATCTGGCCAGCGAACGGATGAGGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9202.49 Da
• Isoelectric Point: 4.0785

>AT93798 WP_032693964.1 NZ_CP026276:162487-162738 [Klebsiella oxytoca]
MPNIILSDTSASISELKKNPMATVSAGDGFPVAILNRNQPAFYCIPAELYEKMLDALDDQELAKLVSERSNQRLLDVDLD
TYL

Nucleotide

• Length: 252 bp

>AT93798 NZ_CP026276:162487-162738 [Klebsiella oxytoca]
ATGCCCAACATTATTCTGAGTGACACAAGCGCGAGCATCAGCGAACTTAAAAAAAACCCGATGGCGACGGTCAGTGCCGG
CGACGGCTTCCCGGTCGCTATTCTGAACCGTAATCAGCCCGCTTTTTACTGCATCCCGGCAGAGCTTTACGAGAAGATGC
TCGATGCCCTGGATGATCAGGAACTGGCAAAGCTGGTTTCAGAACGCAGTAACCAGCGGCTGCTTGACGTGGATCTGGAC
ACGTATCTATGA

Structures

Toxin

• AlphaFold DB: A0A6M4NRI2