Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3700131..3700750 Replicon chromosome
Accession NZ_CP026275
Organism Klebsiella oxytoca strain KONIH5

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag C2U46_RS18015 Protein ID WP_004099646.1
Coordinates 3700131..3700349 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag C2U46_RS18020 Protein ID WP_004129911.1
Coordinates 3700376..3700750 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U46_RS17985 3696176..3696439 + 264 WP_004848323.1 type B 50S ribosomal protein L31 -
C2U46_RS17990 3696439..3696579 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
C2U46_RS17995 3696576..3697274 - 699 WP_014228203.1 GNAT family N-acetyltransferase -
C2U46_RS18000 3697376..3698830 + 1455 WP_032694158.1 PLP-dependent aminotransferase family protein -
C2U46_RS18005 3698805..3699275 - 471 WP_014228205.1 YlaC family protein -
C2U46_RS18010 3699402..3699968 - 567 WP_032694159.1 maltose O-acetyltransferase -
C2U46_RS18015 3700131..3700349 - 219 WP_004099646.1 hemolysin expression modulator Hha Toxin
C2U46_RS18020 3700376..3700750 - 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
C2U46_RS18025 3701231..3704377 - 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit -
C2U46_RS18030 3704400..3705593 - 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T93788 WP_004099646.1 NZ_CP026275:c3700349-3700131 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T93788 NZ_CP026275:c3700349-3700131 [Klebsiella oxytoca]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCTGCGGCCGATCATCGTCTTGCAGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT93788 WP_004129911.1 NZ_CP026275:c3700750-3700376 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT93788 NZ_CP026275:c3700750-3700376 [Klebsiella oxytoca]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGATCCAACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTTGCACTTAATTATAAAATTAAGTACGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References