93786

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3008835..3009411 Replicon chromosome
Accession NZ_CP026275
Organism Klebsiella oxytoca strain KONIH5

Toxin (Protein)


Gene name shpA Uniprot ID A0A1D8JNH2
Locus tag C2U46_RS14750 Protein ID WP_042933975.1
Coordinates 3008835..3009122 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1D8JNF7
Locus tag C2U46_RS14755 Protein ID WP_025108476.1
Coordinates 3009109..3009411 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U46_RS14720 3004176..3004694 - 519 WP_004098240.1 hypothetical protein -
C2U46_RS14725 3004691..3005491 - 801 WP_032693034.1 winged helix-turn-helix domain-containing protein -
C2U46_RS14730 3005637..3006221 - 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -
C2U46_RS14735 3006369..3007151 - 783 WP_032693033.1 NAD(P)H-dependent oxidoreductase -
C2U46_RS14740 3007239..3008147 + 909 WP_032693031.1 LysR family transcriptional regulator -
C2U46_RS14745 3008141..3008584 + 444 WP_032693030.1 FosA family fosfomycin resistance glutathione transferase -
C2U46_RS14750 3008835..3009122 + 288 WP_042933975.1 BrnT family toxin Toxin
C2U46_RS14755 3009109..3009411 + 303 WP_025108476.1 BrnA antitoxin family protein Antitoxin
C2U46_RS14760 3009532..3010875 - 1344 WP_032693026.1 APC family permease -
C2U46_RS14765 3010872..3011783 - 912 WP_032693024.1 acetamidase/formamidase family protein -
C2U46_RS14770 3012235..3012570 - 336 WP_014227760.1 endoribonuclease SymE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11239.69 Da        Isoelectric Point: 8.5899

>T93786 WP_042933975.1 NZ_CP026275:3008835-3009122 [Klebsiella oxytoca]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Download         Length: 288 bp

>T93786 NZ_CP026275:3008835-3009122 [Klebsiella oxytoca]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTATCGGTC
TCGTTCATGGGGTCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11563.17 Da        Isoelectric Point: 9.7962

>AT93786 WP_025108476.1 NZ_CP026275:3009109-3009411 [Klebsiella oxytoca]
MNMVKHKRDNPDTLTAKRADELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp

>AT93786 NZ_CP026275:3009109-3009411 [Klebsiella oxytoca]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGCCGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8JNH2

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8JNF7

References