Detailed information of TA system TA093780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 68407..69050
• Replicon: plasmid pKPC-4b66
• Accession: NZ_CP026274
• Organism: Klebsiella oxytoca strain KONIH4

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: C2U44_RS34035
• Protein ID: WP_016236302.1
• Coordinates: 68407..68823 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: C2U44_RS34040
• Protein ID: WP_001261282.1
• Coordinates: 68820..69050 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U44_RS34015	63775..64125	+	351	WP_004187110.1	DUF305 domain-containing protein	-
C2U44_RS34020	64311..65225	-	915	WP_170873316.1	HNH endonuclease	-
C2U44_RS34025	65764..66786	-	1023	WP_000361404.1	helicase UvrD	-
C2U44_RS34030	66771..68333	-	1563	WP_004206609.1	AAA family ATPase	-
C2U44_RS34035	68407..68823	-	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
C2U44_RS34040	68820..69050	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C2U44_RS35640	69007..69510	+	504	Protein_79	hypothetical protein	-
C2U44_RS34050	69620..69973	+	354	WP_032700769.1	hypothetical protein	-
C2U44_RS34055	70036..70644	+	609	WP_032700768.1	hypothetical protein	-
C2U44_RS34060	70873..71202	+	330	WP_032700767.1	hypothetical protein	-
C2U44_RS34065	71199..71987	+	789	WP_047722707.1	site-specific integrase	-
C2U44_RS34070	72044..72301	-	258	WP_032700766.1	hypothetical protein	-
C2U44_RS35645	72430..72543	-	114	WP_162180131.1	hypothetical protein	-
C2U44_RS34075	72862..73020	-	159	Protein_86	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / dfrA14	-	1..235655	235655

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T93780 WP_016236302.1 NZ_CP026274:c68823-68407 [Klebsiella oxytoca]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T93780 NZ_CP026274:c68823-68407 [Klebsiella oxytoca]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT93780 WP_001261282.1 NZ_CP026274:c69050-68820 [Klebsiella oxytoca]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT93780 NZ_CP026274:c69050-68820 [Klebsiella oxytoca]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAAGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3