Detailed information of TA system TA093779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 35130..35866
• Replicon: plasmid pKPC-4b66
• Accession: NZ_CP026274
• Organism: Klebsiella oxytoca strain KONIH4

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J5Q928
• Locus tag: C2U44_RS33845
• Protein ID: WP_004098919.1
• Coordinates: 35130..35612 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A2J5Q9C0
• Locus tag: C2U44_RS33850
• Protein ID: WP_004098921.1
• Coordinates: 35600..35866 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U44_RS33820	30989..31693	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
C2U44_RS33825	31757..31912	-	156	Protein_36	IS3 family transposase	-
C2U44_RS33830	31902..32684	+	783	Protein_37	hypothetical protein	-
C2U44_RS33835	32725..33516	-	792	WP_003032641.1	tetratricopeptide repeat protein	-
C2U44_RS33840	33575..34921	-	1347	WP_077255298.1	ISNCY family transposase	-
C2U44_RS33845	35130..35612	-	483	WP_004098919.1	GNAT family N-acetyltransferase	Toxin
C2U44_RS33850	35600..35866	-	267	WP_004098921.1	DUF1778 domain-containing protein	Antitoxin
C2U44_RS33855	36042..36296	-	255	WP_004118702.1	hypothetical protein	-
C2U44_RS33860	36372..36629	-	258	WP_004098928.1	hypothetical protein	-
C2U44_RS33865	36678..36881	-	204	WP_004098931.1	hemolysin expression modulator Hha	-
C2U44_RS33870	36912..37280	-	369	WP_042946273.1	hypothetical protein	-
C2U44_RS33875	37324..37818	-	495	WP_013023776.1	hypothetical protein	-
C2U44_RS33880	37849..38415	-	567	WP_009654312.1	hypothetical protein	-
C2U44_RS33885	38412..38675	-	264	WP_009310051.1	hypothetical protein	-
C2U44_RS33890	38965..39543	+	579	WP_009654302.1	hypothetical protein	-
C2U44_RS33895	39682..40683	-	1002	WP_009654304.1	Abi family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / dfrA14	-	1..235655	235655

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T93779 WP_004098919.1 NZ_CP026274:c35612-35130 [Klebsiella oxytoca]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T93779 NZ_CP026274:c35612-35130 [Klebsiella oxytoca]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10105.49 Da
• Isoelectric Point: 4.5141

>AT93779 WP_004098921.1 NZ_CP026274:c35866-35600 [Klebsiella oxytoca]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT93779 NZ_CP026274:c35866-35600 [Klebsiella oxytoca]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTAGAGATTGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A2J5Q928

Antitoxin

• AlphaFold DB: A0A2J5Q9C0