Detailed information of TA system TA093778

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 96929..97506
• Replicon: plasmid pKOX-ea2b
• Accession: NZ_CP026273
• Organism: Klebsiella oxytoca strain KONIH4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C2U44_RS33185
• Protein ID: WP_023286013.1
• Coordinates: 96929..97261 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: C2U44_RS33190
• Protein ID: WP_023286014.1
• Coordinates: 97261..97506 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U44_RS33150	93156..94016	-	861	WP_045268442.1	hypothetical protein	-
C2U44_RS33155	94186..94392	-	207	WP_045268441.1	hypothetical protein	-
C2U44_RS33160	94382..94696	-	315	WP_103433639.1	hypothetical protein	-
C2U44_RS33165	94746..95165	-	420	WP_052684049.1	hypothetical protein	-
C2U44_RS33170	95257..95559	-	303	WP_023286010.1	hypothetical protein	-
C2U44_RS33175	95571..96209	-	639	WP_052684048.1	hypothetical protein	-
C2U44_RS33180	96335..96655	-	321	WP_023286012.1	hypothetical protein	-
C2U44_RS33185	96929..97261	-	333	WP_023286013.1	endoribonuclease MazF	Toxin
C2U44_RS33190	97261..97506	-	246	WP_023286014.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C2U44_RS33195	97587..98144	-	558	WP_045268440.1	hypothetical protein	-
C2U44_RS33200	98214..98489	-	276	WP_045268439.1	hypothetical protein	-
C2U44_RS33205	98557..99243	-	687	WP_052684047.1	hypothetical protein	-
C2U44_RS33210	99959..100216	-	258	WP_045268511.1	hypothetical protein	-
C2U44_RS33215	100608..101294	-	687	WP_052684046.1	hypothetical protein	-
C2U44_RS33220	101291..101728	-	438	WP_045268438.1	hypothetical protein	-
C2U44_RS33225	101728..102390	-	663	WP_045268437.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..179680	179680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11943.82 Da
• Isoelectric Point: 8.7208

>T93778 WP_023286013.1 NZ_CP026273:c97261-96929 [Klebsiella oxytoca]
MVKRYVPDAGDLIWIDFDPVAGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTQVKGYPFEVTLSGSKEGVALSDQITCV
DWRARKVTQKSTVSKSELAEIRAKAKALIG

Nucleotide

• Length: 333 bp

>T93778 NZ_CP026273:c97261-96929 [Klebsiella oxytoca]
ATGGTAAAGCGTTATGTTCCAGATGCGGGCGATCTTATCTGGATTGATTTTGACCCGGTAGCCGGGCATGAACAGGGCGG
ACACCGCCCTGCTGTTGTTTTGAGTCCTTTTGCCTACAACAACAAAGTTGGCCTCCTTCTGTGTGTTCCATGCACGACGC
AAGTTAAGGGATACCCCTTTGAGGTCACGTTGTCCGGCAGCAAAGAAGGCGTAGCCCTGTCAGATCAGATAACCTGCGTT
GACTGGCGAGCCAGAAAAGTGACCCAAAAATCCACAGTAAGTAAGTCGGAGCTTGCAGAAATCCGGGCCAAAGCAAAGGC
GTTGATTGGGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8742.11 Da
• Isoelectric Point: 5.0176

>AT93778 WP_023286014.1 NZ_CP026273:c97506-97261 [Klebsiella oxytoca]
MAQVIVKKWGNSPSVRLPVAVMKAASLNVDDTVDIEVEDGRIIIVPARPKEYSLDALLSGISSDNLHDKVDFGPAKGKEL
L

Nucleotide

• Length: 246 bp

>AT93778 NZ_CP026273:c97506-97261 [Klebsiella oxytoca]
ATGGCTCAGGTAATAGTAAAAAAATGGGGTAACAGCCCGTCAGTCCGGTTGCCAGTAGCAGTGATGAAAGCGGCATCGCT
AAATGTCGATGATACTGTCGATATTGAAGTAGAAGATGGTCGTATCATCATCGTCCCTGCAAGACCGAAAGAATATTCCC
TTGATGCGCTGCTGTCTGGTATTTCCTCCGATAATCTGCATGACAAAGTGGATTTTGGCCCTGCGAAAGGTAAGGAATTG
CTCTGA