Detailed information of TA system TA093776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 5490703..5491279
• Replicon: chromosome
• Accession: NZ_CP026269
• Organism: Klebsiella oxytoca strain KONIH4

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: C2U44_RS27095
• Protein ID: WP_038424021.1
• Coordinates: 5490992..5491279 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0H3H591
• Locus tag: C2U44_RS27090
• Protein ID: WP_014227757.1
• Coordinates: 5490703..5491005 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U44_RS27075	5487543..5487878	+	336	WP_014227760.1	endoribonuclease SymE	-
C2U44_RS27080	5488331..5489242	+	912	WP_032719936.1	acetamidase/formamidase family protein	-
C2U44_RS27085	5489239..5490582	+	1344	WP_032719935.1	APC family permease	-
C2U44_RS27090	5490703..5491005	-	303	WP_014227757.1	BrnA antitoxin family protein	Antitoxin
C2U44_RS27095	5490992..5491279	-	288	WP_038424021.1	BrnT family toxin	Toxin
C2U44_RS27100	5491529..5491972	-	444	WP_014837312.1	FosA family fosfomycin resistance glutathione transferase	-
C2U44_RS27105	5491966..5492874	-	909	WP_014837311.1	LysR family transcriptional regulator	-
C2U44_RS27110	5492962..5493744	+	783	WP_103433588.1	NAD(P)H-dependent oxidoreductase	-
C2U44_RS27115	5493892..5494476	+	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
C2U44_RS27120	5494622..5495422	+	801	WP_014227752.1	winged helix-turn-helix domain-containing protein	-
C2U44_RS27125	5495419..5495937	+	519	WP_042944214.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11253.72 Da
• Isoelectric Point: 8.5899

>T93776 WP_038424021.1 NZ_CP026269:c5491279-5490992 [Klebsiella oxytoca]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T93776 NZ_CP026269:c5491279-5490992 [Klebsiella oxytoca]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAGGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCGGCC
TCGTTCATGGGATCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11607.18 Da
• Isoelectric Point: 9.4429

>AT93776 WP_014227757.1 NZ_CP026269:c5491005-5490703 [Klebsiella oxytoca]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT93776 NZ_CP026269:c5491005-5490703 [Klebsiella oxytoca]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3H591