Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4768914..4769533 Replicon chromosome
Accession NZ_CP026269
Organism Klebsiella oxytoca strain KONIH4

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag C2U44_RS23660 Protein ID WP_004099646.1
Coordinates 4769315..4769533 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag C2U44_RS23655 Protein ID WP_004129911.1
Coordinates 4768914..4769288 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U44_RS23645 4764071..4765264 + 1194 WP_042944463.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C2U44_RS23650 4765287..4768433 + 3147 WP_103433585.1 multidrug efflux RND transporter permease subunit AcrB -
C2U44_RS23655 4768914..4769288 + 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
C2U44_RS23660 4769315..4769533 + 219 WP_004099646.1 hemolysin expression modulator Hha Toxin
C2U44_RS23665 4769696..4770262 + 567 WP_042944461.1 maltose O-acetyltransferase -
C2U44_RS23670 4770389..4770859 + 471 WP_014228205.1 YlaC family protein -
C2U44_RS23675 4770834..4772288 - 1455 WP_042944459.1 PLP-dependent aminotransferase family protein -
C2U44_RS23680 4772390..4773088 + 699 WP_014228203.1 GNAT family N-acetyltransferase -
C2U44_RS23685 4773085..4773225 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
C2U44_RS23690 4773225..4773488 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T93773 WP_004099646.1 NZ_CP026269:4769315-4769533 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T93773 NZ_CP026269:4769315-4769533 [Klebsiella oxytoca]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCTGCGGCCGATCATCGTCTTGCAGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT93773 WP_004129911.1 NZ_CP026269:4768914-4769288 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT93773 NZ_CP026269:4768914-4769288 [Klebsiella oxytoca]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGATCCAACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTTGCACTTAATTATAAAATTAAGTACGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References