Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2176783..2177373 Replicon chromosome
Accession NZ_CP026269
Organism Klebsiella oxytoca strain KONIH4

Toxin (Protein)


Gene name pemK Uniprot ID A0A0H3HDW1
Locus tag C2U44_RS10820 Protein ID WP_014229979.1
Coordinates 2177041..2177373 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag C2U44_RS10815 Protein ID WP_004852307.1
Coordinates 2176783..2177040 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U44_RS10780 2172039..2172647 - 609 WP_162763184.1 glutathione S-transferase family protein -
C2U44_RS10785 2172824..2173750 + 927 WP_042945600.1 LysR family transcriptional regulator -
C2U44_RS10790 2173788..2174786 - 999 WP_014229982.1 aldo/keto reductase -
C2U44_RS10795 2174887..2175801 + 915 WP_042945598.1 LysR family transcriptional regulator -
C2U44_RS10800 2175992..2176072 - 81 Protein_2047 molybdopterin-binding protein -
C2U44_RS10815 2176783..2177040 + 258 WP_004852307.1 antitoxin Antitoxin
C2U44_RS10820 2177041..2177373 + 333 WP_014229979.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
C2U44_RS10830 2177696..2179153 + 1458 WP_014229916.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
C2U44_RS10840 2180116..2181570 - 1455 WP_014229909.1 AMP nucleosidase -
C2U44_RS10845 2181703..2181960 - 258 WP_014229908.1 histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11740.57 Da        Isoelectric Point: 10.1863

>T93763 WP_014229979.1 NZ_CP026269:2177041-2177373 [Klebsiella oxytoca]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T93763 NZ_CP026269:2177041-2177373 [Klebsiella oxytoca]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGTGGAAATTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCAGGGACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAACGGCAAGCGGCTGGAAAGCATACCAGACGCTGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTAAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT93763 WP_004852307.1 NZ_CP026269:2176783-2177040 [Klebsiella oxytoca]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT93763 NZ_CP026269:2176783-2177040 [Klebsiella oxytoca]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCTCATTATTCTC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCTCCTGCGGTG
GGCAAGGAGATCCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3HDW1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References