Detailed information of TA system TA093762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1382666..1383219
• Replicon: chromosome
• Accession: NZ_CP026269
• Organism: Klebsiella oxytoca strain KONIH4

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: C2U44_RS06950
• Protein ID: WP_016157234.1
• Coordinates: 1382905..1383219 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: C2U44_RS06945
• Protein ID: WP_016157235.1
• Coordinates: 1382666..1382902 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U44_RS06930	1379216..1379722	-	507	WP_032931759.1	hypothetical protein	-
C2U44_RS06935	1379719..1380927	-	1209	WP_042945865.1	hypothetical protein	-
C2U44_RS06940	1381399..1382343	+	945	WP_042945864.1	DUF1738 domain-containing protein	-
C2U44_RS06945	1382666..1382902	+	237	WP_016157235.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
C2U44_RS06950	1382905..1383219	+	315	WP_016157234.1	CcdB family protein	Toxin
C2U44_RS06955	1383216..1383968	-	753	WP_016157233.1	hypothetical protein	-
C2U44_RS06960	1383977..1385713	-	1737	WP_042945862.1	type IV secretory system conjugative DNA transfer family protein	-
C2U44_RS06975	1386490..1386795	-	306	WP_016157231.1	hypothetical protein	-
C2U44_RS35095	1386842..1387072	-	231	Protein_1315	kikA from plasmid origin	-
C2U44_RS06985	1387222..1388202	-	981	WP_016947617.1	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1312281..1424231	111950
	-	flank	IS/Tn	-	-	1387222..1388202	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11415.29 Da
• Isoelectric Point: 8.9025

>T93762 WP_016157234.1 NZ_CP026269:1382905-1383219 [Klebsiella oxytoca]
MQFTVYGNTGKSAVYPLLLDVTSDIIGQLNRRIVIPLLPAGRYPAGRRPERLVPVVRLTDGQEYAVMTHELASVPVRALG
AVFCDASQYRTQVKAAIDFLIDGI

Nucleotide

• Length: 315 bp

>T93762 NZ_CP026269:1382905-1383219 [Klebsiella oxytoca]
ATGCAGTTCACCGTATACGGAAACACCGGTAAAAGCGCCGTTTACCCTCTGCTGCTTGATGTCACGAGCGATATTATCGG
ACAACTGAACCGCCGGATCGTGATCCCGCTGCTGCCTGCTGGCAGGTATCCGGCAGGACGCCGCCCGGAGCGTCTTGTCC
CTGTCGTCAGACTGACAGACGGTCAGGAATACGCGGTCATGACCCACGAACTGGCGAGTGTCCCTGTCCGGGCGCTGGGA
GCGGTTTTCTGCGATGCGTCTCAATACCGCACTCAGGTGAAGGCCGCCATCGATTTCCTCATTGACGGCATTTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9044.08 Da
• Isoelectric Point: 6.2206

>AT93762 WP_016157235.1 NZ_CP026269:1382666-1382902 [Klebsiella oxytoca]
MTAKQRSTQSVTMTVERALLIRAREAGINLSATLTTALDAELRHYEAKKWQEENREAIDALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT93762 NZ_CP026269:1382666-1382902 [Klebsiella oxytoca]
ATGACCGCGAAACAACGCAGCACGCAGAGTGTGACCATGACGGTAGAGCGCGCCTTACTGATCCGGGCACGTGAGGCAGG
CATTAACCTCAGTGCGACCCTGACAACCGCCCTGGATGCGGAGCTTCGCCACTATGAAGCGAAAAAATGGCAGGAAGAGA
ACCGGGAGGCCATCGACGCATTAAATCGTTTCCATGATGAGCATGGCTGTTTCAGTGATGAATACCGGACATTTTAA