Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 130533..131443 Replicon plasmid pCFR-9161
Accession NZ_CP026237
Organism Citrobacter freundii complex sp. CFNIH3

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag C2U38_RS27825 Protein ID WP_004026354.1
Coordinates 130973..131443 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag C2U38_RS27820 Protein ID WP_004181895.1
Coordinates 130533..130976 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U38_RS27785 125626..126426 + 801 WP_001515712.1 hypothetical protein -
C2U38_RS27790 126520..127500 + 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -
C2U38_RS27800 127720..128700 + 981 WP_103284490.1 IS5-like element ISKpn26 family transposase -
C2U38_RS27810 128847..129161 + 315 Protein_122 translesion error-prone DNA polymerase V autoproteolytic subunit -
C2U38_RS27815 129161..130432 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
C2U38_RS27820 130533..130976 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
C2U38_RS27825 130973..131443 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
C2U38_RS27830 131553..131813 - 261 WP_004026352.1 hypothetical protein -
C2U38_RS27840 132512..132904 + 393 Protein_127 transposase -
C2U38_RS27845 132855..133943 - 1089 WP_001515707.1 IS481-like element ISKpn28 family transposase -
C2U38_RS27850 134072..135604 - 1533 WP_001515706.1 IS3-like element ISKpn38 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..235027 235027
- inside IScluster/Tn - - 122951..139388 16437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T93750 WP_004026354.1 NZ_CP026237:130973-131443 [Citrobacter freundii complex sp. CFNIH3]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T93750 NZ_CP026237:130973-131443 [Citrobacter freundii complex sp. CFNIH3]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT93750 WP_004181895.1 NZ_CP026237:130533-130976 [Citrobacter freundii complex sp. CFNIH3]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT93750 NZ_CP026237:130533-130976 [Citrobacter freundii complex sp. CFNIH3]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References