Detailed information of TA system TA093749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 119484..120127
• Replicon: plasmid pCFR-9161
• Accession: NZ_CP026237
• Organism: Citrobacter freundii complex sp. CFNIH3

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: C2U38_RS27745
• Protein ID: WP_053263822.1
• Coordinates: 119711..120127 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: C2U38_RS27740
• Protein ID: WP_001261282.1
• Coordinates: 119484..119714 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U38_RS27710	114997..115263	-	267	WP_024144810.1	type II toxin-antitoxin system ParD family antitoxin	-
C2U38_RS27715	115494..117167	-	1674	WP_000026497.1	hypothetical protein	-
C2U38_RS27725	117732..118541	-	810	WP_000654269.1	hypothetical protein	-
C2U38_RS27730	118559..118921	-	363	WP_000533745.1	hypothetical protein	-
C2U38_RS28710	119105..119527	-	423	WP_162534719.1	hypothetical protein	-
C2U38_RS27740	119484..119714	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C2U38_RS27745	119711..120127	+	417	WP_053263822.1	type II toxin-antitoxin system VapC family toxin	Toxin
C2U38_RS27750	120201..121763	+	1563	WP_001128475.1	AAA family ATPase	-
C2U38_RS27755	121748..122770	+	1023	WP_000361404.1	helicase UvrD	-
C2U38_RS27765	123699..123920	+	222	Protein_116	transposase	-
C2U38_RS27775	124069..124386	-	318	Protein_117	hypothetical protein	-
C2U38_RS27780	124383..124868	-	486	WP_000045622.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..235027	235027
	-	inside	IScluster/Tn	-	-	122951..139388	16437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15090.56 Da
• Isoelectric Point: 7.7521

>T93749 WP_053263822.1 NZ_CP026237:119711-120127 [Citrobacter freundii complex sp. CFNIH3]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T93749 NZ_CP026237:119711-120127 [Citrobacter freundii complex sp. CFNIH3]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCGCCGCGCCACGTCCAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGACCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT93749 WP_001261282.1 NZ_CP026237:119484-119714 [Citrobacter freundii complex sp. CFNIH3]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT93749 NZ_CP026237:119484-119714 [Citrobacter freundii complex sp. CFNIH3]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3