Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4997129..4997745 Replicon chromosome
Accession NZ_CP026235
Organism Citrobacter freundii complex sp. CFNIH3

Toxin (Protein)


Gene name vapC Uniprot ID A0A2I8SCX0
Locus tag C2U38_RS25125 Protein ID WP_049269424.1
Coordinates 4997129..4997503 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID R8WIS4
Locus tag C2U38_RS25130 Protein ID WP_016155181.1
Coordinates 4997503..4997745 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U38_RS25110 4994632..4995534 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
C2U38_RS25115 4995531..4996166 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
C2U38_RS25120 4996163..4997092 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
C2U38_RS25125 4997129..4997503 - 375 WP_049269424.1 type II toxin-antitoxin system VapC family toxin Toxin
C2U38_RS25130 4997503..4997745 - 243 WP_016155181.1 ribbon-helix-helix domain-containing protein Antitoxin
C2U38_RS25135 4997951..4998859 + 909 WP_049269423.1 alpha/beta hydrolase -
C2U38_RS25140 4998924..4999166 + 243 WP_016155179.1 type II toxin-antitoxin system ParD family antitoxin -
C2U38_RS25145 4999159..4999446 + 288 WP_049269420.1 type II toxin-antitoxin system RelE/ParE family toxin -
C2U38_RS25150 4999452..5000393 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
C2U38_RS25155 5000438..5000875 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
C2U38_RS25160 5000872..5001744 - 873 WP_019077938.1 virulence factor BrkB family protein -
C2U38_RS25165 5001738..5002267 - 530 Protein_4720 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13650.78 Da        Isoelectric Point: 8.5373

>T93747 WP_049269424.1 NZ_CP026235:c4997503-4997129 [Citrobacter freundii complex sp. CFNIH3]
MATGSALFDTNILIDLFSGRSEAKLAIETWPPQNAISLITWMELMVGAKKYHQESRTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTRDFAGISGVVTPYTL

Download         Length: 375 bp

>T93747 NZ_CP026235:c4997503-4997129 [Citrobacter freundii complex sp. CFNIH3]
ATGGCCACAGGATCTGCGCTTTTTGATACCAACATCCTCATCGACCTGTTTAGCGGGCGAAGTGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAACGCAATCAGCCTCATTACATGGATGGAGTTGATGGTGGGCGCAAAAAAATATCATCAGG
AATCTCGCACACGCGTTGCGCTAGGCGCTTTTAATGTGATAGGTGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGGCAGGAATATGGGATGAAATTGCCGGATGCCATCATTTTGGCCACTGCGCAGATTCATCGTCTTACACTGGTTACGCG
TAATACCAGGGATTTTGCAGGCATATCCGGCGTGGTCACACCGTATACGTTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9437.72 Da        Isoelectric Point: 4.3331

>AT93747 WP_016155181.1 NZ_CP026235:c4997745-4997503 [Citrobacter freundii complex sp. CFNIH3]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVDYQRKLREEW

Download         Length: 243 bp

>AT93747 NZ_CP026235:c4997745-4997503 [Citrobacter freundii complex sp. CFNIH3]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGAAGTGATCAAACGTCTTGATGATCTCAAGGT
GCAGCGTAATCTGCCGCGGGCCGAGCTTTTGCGGGAGGCTGTTGAACAGTATCTTGAGAGGCAGGATCAAGCTGAAACGA
CAATTTCACGTGCTCTTGGGCTATGGCAAGGTTGTGAGGAAGATGGCGTTGATTATCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8SCX0

Antitoxin

Source ID Structure
AlphaFold DB R8WIS4

References