Detailed information of TA system TA093745

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4139252..4139906
• Replicon: chromosome
• Accession: NZ_CP026235
• Organism: Citrobacter freundii complex sp. CFNIH3

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R8WN60
• Locus tag: C2U38_RS20640
• Protein ID: WP_016154348.1
• Coordinates: 4139252..4139659 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: C2U38_RS20645
• Protein ID: WP_016154349.1
• Coordinates: 4139640..4139906 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U38_RS20620	4135144..4136877	-	1734	WP_103284192.1	single-stranded-DNA-specific exonuclease RecJ	-
C2U38_RS20625	4136883..4137596	-	714	WP_075847456.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
C2U38_RS20630	4137620..4138516	-	897	WP_016154346.1	site-specific tyrosine recombinase XerD	-
C2U38_RS20635	4138630..4139151	+	522	WP_016154347.1	flavodoxin FldB	-
C2U38_RS20640	4139252..4139659	-	408	WP_016154348.1	protein YgfX	Toxin
C2U38_RS20645	4139640..4139906	-	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
C2U38_RS20655	4140164..4141144	+	981	WP_016154350.1	tRNA-modifying protein YgfZ	-
C2U38_RS20660	4141224..4141883	-	660	WP_016154351.1	hemolysin III family protein	-
C2U38_RS20665	4142047..4142358	-	312	WP_016154352.1	N(4)-acetylcytidine aminohydrolase	-
C2U38_RS20670	4142411..4143139	+	729	WP_103284472.1	MurR/RpiR family transcriptional regulator	-
C2U38_RS20675	4143260..4144693	+	1434	WP_016154353.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15850.66 Da
• Isoelectric Point: 11.2845

>T93745 WP_016154348.1 NZ_CP026235:c4139659-4139252 [Citrobacter freundii complex sp. CFNIH3]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T93745 NZ_CP026235:c4139659-4139252 [Citrobacter freundii complex sp. CFNIH3]
GTGGTCCAGTGGCAATCTGATTTACGCGTTTCATGGCGCGCGCAATGGCTTTCGTTGCTCATTCATGGGCTGGTCGCGGT
ATTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTCTGCTCTCTCTGGTGGTGTTCGATAGCG
TCCGTAGCCAGCGCCGAATCAATGCCTGTCAGGGCGAGATTCGGCTGTTGATGGACGGTCGTCTGCGCTGGCAAGGGCAA
GAGTGGACTATTGCTAGCGCACCCTGGATGGTCAAAACAGGCATGATGTTGCGTTTACGTTCCGATGCCGGAAAACGTCA
GCATTTGTGGCTGGCGGCAGACAGTATGGATGACGCGGAGTGGCGGGATTTGCGGCGGTTAATACTGCAACAGGCGAAGC
AGGGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT93745 WP_016154349.1 NZ_CP026235:c4139906-4139640 [Citrobacter freundii complex sp. CFNIH3]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT93745 NZ_CP026235:c4139906-4139640 [Citrobacter freundii complex sp. CFNIH3]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGTCGCGGTATGCGTGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGATGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGACT
TATTTAACTGGTTGATGAATCATGGGAAACCGGCTGATGCTCAGCTAGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: R8WN60

Antitoxin

• AlphaFold DB: R8WMJ6