Detailed information of TA system TA093744

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3809539..3810206
• Replicon: chromosome
• Accession: NZ_CP026235
• Organism: Citrobacter freundii complex sp. CFNIH3

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A2I8SC83
• Locus tag: C2U38_RS19065
• Protein ID: WP_054626383.1
• Coordinates: 3809877..3810206 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A2I8SA04
• Locus tag: C2U38_RS19060
• Protein ID: WP_054626382.1
• Coordinates: 3809539..3809856 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U38_RS19020	3805534..3805773	+	240	WP_054626379.1	DUF905 domain-containing protein	-
C2U38_RS19025	3805857..3806108	+	252	Protein_3588	antirestriction protein	-
C2U38_RS19030	3806116..3807021	-	906	Protein_3589	IS3-like element ISCfr6 family transposase	-
C2U38_RS19035	3807031..3808182	-	1152	WP_001254932.1	IS30-like element IS30 family transposase	-
C2U38_RS19040	3808250..3808552	-	303	Protein_3591	transposase	-
C2U38_RS19045	3808593..3808799	+	207	Protein_3592	antirestriction protein	-
C2U38_RS19050	3808815..3809291	+	477	WP_054626380.1	RadC family protein	-
C2U38_RS19055	3809300..3809521	+	222	WP_054626381.1	DUF987 domain-containing protein	-
C2U38_RS19060	3809539..3809856	+	318	WP_054626382.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C2U38_RS19065	3809877..3810206	+	330	WP_054626383.1	TA system toxin CbtA family protein	Toxin
C2U38_RS19070	3810603..3810785	+	183	WP_016157242.1	hypothetical protein	-
C2U38_RS19075	3810976..3811722	-	747	WP_016157243.1	SDR family oxidoreductase	-
C2U38_RS19080	3811793..3812482	-	690	WP_016157244.1	YafY family transcriptional regulator	-
C2U38_RS19085	3812939..3813295	+	357	WP_016157245.1	hypothetical protein	-
C2U38_RS19090	3813354..3814787	-	1434	WP_103284129.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3781594..3813295	31701

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12324.29 Da
• Isoelectric Point: 7.1642

>T93744 WP_054626383.1 NZ_CP026235:3809877-3810206 [Citrobacter freundii complex sp. CFNIH3]
MKTLPATTPQAAKLCLPPVAVWQMLLTRLLETHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDRNGF
SWQEQTPYISVVDILRARRSTGLLKNNVK

Nucleotide

• Length: 330 bp

>T93744 NZ_CP026235:3809877-3810206 [Citrobacter freundii complex sp. CFNIH3]
ATGAAAACTTTACCTGCAACAACTCCGCAGGCGGCGAAGCTATGTCTGCCGCCCGTGGCCGTCTGGCAAATGTTACTGAC
ACGCCTGCTGGAAACGCACTATGGCCTGACACTGAACGACACGCCATTCAGTGATGAAACTGTTATTAAGGAACATATTG
ATGCCGGGATCACCCTGGCCGATGCAGTCAACTTTCTGGTGGAAAAATACGAACTGGTTCGTATCGACAGGAATGGATTT
TCGTGGCAAGAACAAACCCCGTATATTTCCGTAGTGGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAAATAA
CGTGAAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11741.53 Da
• Isoelectric Point: 7.0161

>AT93744 WP_054626382.1 NZ_CP026235:3809539-3809856 [Citrobacter freundii complex sp. CFNIH3]
MSKITWGLQRNITPHLGARLVQEGNRLHYLADRASITGKISDAECLKLDVAFPHFISQMELMLTTGEMNPCHAHCVILYH
NGFTCEADTLGSCGYVYVAVYPTQR

Nucleotide

• Length: 318 bp

>AT93744 NZ_CP026235:3809539-3809856 [Citrobacter freundii complex sp. CFNIH3]
ATGAGCAAAATCACATGGGGTCTGCAGCGAAATATCACGCCACACCTGGGAGCCCGTCTGGTGCAGGAGGGGAACCGGCT
GCATTATCTGGCTGATCGGGCCAGTATCACCGGTAAGATCAGTGACGCCGAATGTCTTAAGCTGGATGTGGCATTTCCGC
ATTTTATCAGTCAGATGGAATTGATGCTGACCACCGGTGAAATGAACCCTTGCCATGCCCACTGCGTCATCCTGTATCAC
AACGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACGTCGCCGTTTATCCCACTCAGCGCTAA

Structures

Toxin

• AlphaFold DB: A0A2I8SC83

Antitoxin

• AlphaFold DB: A0A2I8SA04