Detailed information of TA system TA093734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2351745..2352381
• Replicon: chromosome
• Accession: NZ_CP026235
• Organism: Citrobacter freundii complex sp. CFNIH3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2I8S6E9
• Locus tag: C2U38_RS11775
• Protein ID: WP_049259794.1
• Coordinates: 2351745..2351933 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C2U38_RS11780
• Protein ID: WP_146053235.1
• Coordinates: 2351965..2352381 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U38_RS11755	2348523..2348753	-	231	WP_016156585.1	DUF2554 family protein	-
C2U38_RS11760	2348943..2349068	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
C2U38_RS11765	2349068..2350078	-	1011	WP_103283796.1	cytochrome d ubiquinol oxidase subunit II	-
C2U38_RS11770	2350078..2351481	-	1404	WP_016153156.1	cytochrome ubiquinol oxidase subunit I	-
C2U38_RS11775	2351745..2351933	+	189	WP_049259794.1	type II toxin-antitoxin system HicA family toxin	Toxin
C2U38_RS11780	2351965..2352381	+	417	WP_146053235.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C2U38_RS11785	2352474..2353883	+	1410	WP_103283798.1	PLP-dependent aminotransferase family protein	-
C2U38_RS11790	2354213..2355358	+	1146	WP_016153154.1	ABC transporter substrate-binding protein	-
C2U38_RS11795	2355375..2356391	+	1017	WP_016153153.1	ABC transporter ATP-binding protein	-
C2U38_RS11800	2356392..2357336	+	945	WP_016153152.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7102.16 Da
• Isoelectric Point: 11.5775

>T93734 WP_049259794.1 NZ_CP026235:2351745-2351933 [Citrobacter freundii complex sp. CFNIH3]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKSILKQLGLQSSSI

Nucleotide

• Length: 189 bp

>T93734 NZ_CP026235:2351745-2351933 [Citrobacter freundii complex sp. CFNIH3]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTGGCTGTTACAAACGGCTCAAATCATCTGAAGTTGAG
GTACCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCTGGTGATGAAATAAAAGAAGCCTTGCGAAAATCGATCCTGAAGC
AGCTTGGTTTACAGTCATCATCAATATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15080.33 Da
• Isoelectric Point: 4.7119

>AT93734 WP_146053235.1 NZ_CP026235:2351965-2352381 [Citrobacter freundii complex sp. CFNIH3]
MRYPVNLIPSVEGGFVVSFPDIPEALTQGETRHGALQAAQSALVTAFEFYFDDNEPIPLPSAVGTEGDYVEIPLSIASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSVTML

Nucleotide

• Length: 417 bp

>AT93734 NZ_CP026235:2351965-2352381 [Citrobacter freundii complex sp. CFNIH3]
ATGCGTTATCCCGTTAATCTAATCCCCTCCGTGGAAGGTGGATTCGTGGTTTCTTTCCCGGATATTCCTGAAGCATTAAC
CCAGGGCGAAACCCGTCATGGGGCTTTGCAGGCTGCGCAGTCTGCATTGGTGACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTACCTTCGGCGGTTGGCACTGAAGGTGACTATGTGGAAATCCCCCTGAGCATTGCGTCGAAGGTT
CTTTTGTTAAATGCCTTTCTTGAATCAAAAATCACCCAGCAGGAGCTGGCAAACAGGATCGGCAGGCCTAAGCAGGAAAT
TACCCGTTTGTTCGATTTAAAGCACGCGACAAAAATTGATGCTGTGCAAATAGCTGCACGCGCCTTAGGCAAGGAGCTGT
CAGTAACAATGTTGTAA

Structures

Toxin

• AlphaFold DB: A0A2I8S6E9