Detailed information of TA system TA093731

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1308479..1309251
• Replicon: chromosome
• Accession: NZ_CP026235
• Organism: Citrobacter freundii complex sp. CFNIH3

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2I8S4E2
• Locus tag: C2U38_RS06520
• Protein ID: WP_065805889.1
• Coordinates: 1308862..1309251 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C2U38_RS06515
• Protein ID: WP_175364756.1
• Coordinates: 1308479..1308805 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U38_RS06485	1304925..1305557	-	633	WP_072032909.1	inovirus Gp2 family protein	-
C2U38_RS06490	1306094..1306918	+	825	WP_065805884.1	DUF945 domain-containing protein	-
C2U38_RS06495	1306915..1307172	+	258	WP_044349282.1	DUF905 domain-containing protein	-
C2U38_RS06500	1307252..1307695	+	444	WP_065805885.1	antirestriction protein	-
C2U38_RS06505	1307707..1308186	+	480	WP_065805886.1	DNA repair protein RadC	-
C2U38_RS06510	1308200..1308421	+	222	WP_065805887.1	DUF987 domain-containing protein	-
C2U38_RS06515	1308479..1308805	+	327	WP_175364756.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C2U38_RS06520	1308862..1309251	+	390	WP_065805889.1	TA system toxin CbtA family protein	Toxin
C2U38_RS06525	1309338..1310183	+	846	WP_065805890.1	DUF4942 domain-containing protein	-
C2U38_RS06535	1311086..1312138	-	1053	WP_103283575.1	macro domain-containing protein	-
C2U38_RS06540	1312152..1312799	-	648	WP_103284449.1	DUF4433 domain-containing protein	-
C2U38_RS06545	1312900..1313733	-	834	WP_103283576.1	DUF4942 domain-containing protein	-
C2U38_RS06550	1313847..1314167	-	321	WP_103283577.1	TA system toxin CbtA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1284829..1374117	89288

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14321.40 Da
• Isoelectric Point: 9.9883

>T93731 WP_065805889.1 NZ_CP026235:1308862-1309251 [Citrobacter freundii complex sp. CFNIH3]
MQTKSSPLMRAASSRPSPVSVWQTLLTSLLAQHYGLTLSDTPFSDEQVIQQHIDAGISLVDALNFIVEKYELVRTDRPGF
SILTQSPFITPIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Nucleotide

• Length: 390 bp

>T93731 NZ_CP026235:1308862-1309251 [Citrobacter freundii complex sp. CFNIH3]
ATGCAAACCAAATCATCCCCCCTCATGCGGGCGGCATCGTCACGTCCGTCTCCGGTGAGTGTCTGGCAGACCCTGCTGAC
CAGCCTGCTGGCACAACACTATGGCCTCACGCTTAGCGATACGCCGTTCAGTGATGAACAGGTCATTCAGCAACATATTG
ATGCCGGTATCTCGCTGGTTGATGCCCTGAATTTTATCGTTGAGAAGTATGAACTGGTCCGCACTGACCGCCCAGGCTTC
AGCATCCTGACGCAGTCTCCGTTTATCACACCCATAGATATCCTCCGGGCCAGGAAAGCAACGGGGCTGATGAACCGTGA
CACGTATAAAGAGGTGACGGCTATTACCAGAGGGCAACATCCGCAGGTCAGCGCTCCAGGCAAGCGATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12054.78 Da
• Isoelectric Point: 6.9459

>AT93731 WP_175364756.1 NZ_CP026235:1308479-1308805 [Citrobacter freundii complex sp. CFNIH3]
ISEPWWGLRRSVSPCFGARLVQEGNRLHYLADRANVSGQFCDADLRHLDQAFPILLKQLELMLTSGELNPRHQHCVTLYA
KGLTCEADTLGSHGYVYIAIYPTPVATA

Nucleotide

• Length: 327 bp

>AT93731 NZ_CP026235:1308479-1308805 [Citrobacter freundii complex sp. CFNIH3]
ATCAGCGAACCCTGGTGGGGGCTCAGGCGAAGTGTCTCCCCGTGTTTTGGGGCCCGTCTGGTCCAGGAGGGAAACCGCCT
GCATTACCTGGCTGACCGGGCGAATGTTAGCGGACAGTTCTGTGATGCGGATTTACGCCATCTTGACCAGGCTTTCCCGA
TATTGCTCAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAATCCCCGTCATCAGCACTGCGTCACGCTGTATGCA
AAAGGTCTGACCTGCGAGGCCGATACGCTCGGTTCGCACGGCTATGTTTATATCGCGATTTATCCCACTCCTGTCGCCAC
GGCATAA

Structures

Toxin

• AlphaFold DB: A0A2I8S4E2