Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1197769..1198389 Replicon chromosome
Accession NZ_CP026235
Organism Citrobacter freundii complex sp. CFNIH3

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag C2U38_RS05925 Protein ID WP_002892050.1
Coordinates 1197769..1197987 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag C2U38_RS05930 Protein ID WP_003021733.1
Coordinates 1198015..1198389 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U38_RS05890 1193129..1193758 + 630 WP_103283554.1 hypothetical protein -
C2U38_RS05895 1193823..1194176 + 354 WP_003831033.1 DUF1428 family protein -
C2U38_RS05900 1194228..1195781 - 1554 WP_047416867.1 EAL domain-containing protein -
C2U38_RS05905 1195970..1196230 + 261 WP_016152070.1 type B 50S ribosomal protein L31 -
C2U38_RS05910 1196232..1196372 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
C2U38_RS05915 1196450..1196920 - 471 WP_016152071.1 YlaC family protein -
C2U38_RS05920 1197037..1197588 - 552 WP_103283555.1 maltose O-acetyltransferase -
C2U38_RS05925 1197769..1197987 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
C2U38_RS05930 1198015..1198389 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
C2U38_RS05935 1198877..1202026 - 3150 WP_016152073.1 multidrug efflux RND transporter permease subunit -
C2U38_RS05940 1202049..1203242 - 1194 WP_016152074.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T93730 WP_002892050.1 NZ_CP026235:c1197987-1197769 [Citrobacter freundii complex sp. CFNIH3]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T93730 NZ_CP026235:c1197987-1197769 [Citrobacter freundii complex sp. CFNIH3]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCCTGCGACGTTGTCAGACAATTGACACACTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCACCGCCTTGCTGAAT
TGACTATGAATAAGCTCTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT93730 WP_003021733.1 NZ_CP026235:c1198389-1198015 [Citrobacter freundii complex sp. CFNIH3]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT93730 NZ_CP026235:c1198389-1198015 [Citrobacter freundii complex sp. CFNIH3]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCCACTAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References