Detailed information of TA system TA093728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 596758..597334
• Replicon: chromosome
• Accession: NZ_CP026235
• Organism: Citrobacter freundii complex sp. CFNIH3

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A2I8S420
• Locus tag: C2U38_RS02985
• Protein ID: WP_049269953.1
• Coordinates: 596758..597045 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A2I8S1U6
• Locus tag: C2U38_RS02990
• Protein ID: WP_016155614.1
• Coordinates: 597032..597334 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U38_RS02955	592066..593130	+	1065	WP_049041982.1	DUF2955 domain-containing protein	-
C2U38_RS02960	593405..593767	+	363	WP_046275905.1	DUF2778 domain-containing protein	-
C2U38_RS02965	593767..594273	+	507	WP_047414535.1	hypothetical protein	-
C2U38_RS02970	594274..594765	+	492	WP_016151575.1	type VI secretion system tube protein Hcp	-
C2U38_RS02975	594896..595194	-	299	Protein_558	SymE family type I addiction module toxin	-
C2U38_RS28585	595157..595297	+	141	WP_165500049.1	hypothetical protein	-
C2U38_RS02980	595360..596526	-	1167	WP_019078303.1	restriction endonuclease	-
C2U38_RS02985	596758..597045	+	288	WP_049269953.1	BrnT family toxin	Toxin
C2U38_RS02990	597032..597334	+	303	WP_016155614.1	BrnA antitoxin family protein	Antitoxin
C2U38_RS02995	597642..597920	-	279	Protein_563	ATP-binding domain-containing protein	-
C2U38_RS03000	597991..599111	+	1121	WP_103283453.1	IS3 family transposase	-
C2U38_RS03005	599140..600282	-	1143	Protein_565	AAA family ATPase	-
C2U38_RS03010	600351..601331	+	981	WP_000019445.1	IS5-like element ISKpn26 family transposase	-
C2U38_RS03020	601480..601914	-	435	Protein_567	NERD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	590378..601914	11536

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (42-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11433.87 Da
• Isoelectric Point: 8.6051

>T93728 WP_049269953.1 NZ_CP026235:596758-597045 [Citrobacter freundii complex sp. CFNIH3]
MPMEFEWDANKAQSNHRKHGVRFEDAILVFDDPQHLSRQERYENGEYRWQTIGLVHGIVVILVAHSVRFESRFEVIRIIS
ARKADRKERNRYEHS

Nucleotide

• Length: 288 bp

>T93728 NZ_CP026235:596758-597045 [Citrobacter freundii complex sp. CFNIH3]
ATGCCAATGGAGTTTGAGTGGGATGCAAACAAGGCACAAAGTAACCACCGTAAACATGGTGTTCGCTTTGAGGACGCAAT
ATTGGTTTTCGACGATCCCCAACATCTGTCGCGGCAGGAGCGGTATGAGAACGGTGAGTATCGCTGGCAGACCATTGGTC
TCGTTCATGGCATTGTGGTTATTCTGGTTGCCCATAGCGTTAGGTTTGAAAGTCGCTTTGAAGTCATTCGTATCATCAGC
GCCAGAAAGGCAGATCGTAAAGAGAGGAATCGTTATGAGCATAGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11466.12 Da
• Isoelectric Point: 10.1293

>AT93728 WP_016155614.1 NZ_CP026235:597032-597334 [Citrobacter freundii complex sp. CFNIH3]
MSIVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDKQWSEAIRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT93728 NZ_CP026235:597032-597334 [Citrobacter freundii complex sp. CFNIH3]
ATGAGCATAGTTAAACATAAACGCGGCAGCGTATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
TAAACCGGACGATGATATCGATTACAGCGACATTCCCGTGACCGAAGATAAGCAGTGGTCAGAGGCCATTCGCGGTAAGT
TTTTTCGTCCGTTAAAAACGCAGGCGTCGGTGCGCATTGATGCCGATGTGATGGAATGGCTGAAGCGCCCCGGGAAGGGT
TACCAGACGCGTCTCAATGCCATTTTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• AlphaFold DB: A0A2I8S420

Antitoxin

• AlphaFold DB: A0A2I8S1U6