Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 545561..546240 Replicon chromosome
Accession NZ_CP026235
Organism Citrobacter freundii complex sp. CFNIH3

Toxin (Protein)


Gene name ykfI Uniprot ID A0A2I8S1Q2
Locus tag C2U38_RS02730 Protein ID WP_074398812.1
Coordinates 545899..546240 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A1B7JQF2
Locus tag C2U38_RS02725 Protein ID WP_064547036.1
Coordinates 545561..545878 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U38_RS02700 540772..543927 + 3156 WP_103283434.1 AIDA repeat-containing protein -
C2U38_RS02705 544020..544259 + 240 WP_064547027.1 DUF905 domain-containing protein -
C2U38_RS02710 544362..544820 + 459 WP_064547030.1 antirestriction protein -
C2U38_RS02715 544836..545312 + 477 WP_064547032.1 RadC family protein -
C2U38_RS02720 545321..545542 + 222 WP_064547034.1 DUF987 domain-containing protein -
C2U38_RS02725 545561..545878 + 318 WP_064547036.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
C2U38_RS02730 545899..546240 + 342 WP_074398812.1 TA system toxin CbtA family protein Toxin
C2U38_RS02735 546818..548089 + 1272 WP_103283435.1 integrase arm-type DNA-binding domain-containing protein -
C2U38_RS02740 548136..550889 - 2754 WP_103283436.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 538967..557043 18076


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-111)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12850.68 Da        Isoelectric Point: 8.0800

>T93727 WP_074398812.1 NZ_CP026235:545899-546240 [Citrobacter freundii complex sp. CFNIH3]
MKPQSATTSRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAFDILRARHATGLLRHSRNNTAI

Download         Length: 342 bp

>T93727 NZ_CP026235:545899-546240 [Citrobacter freundii complex sp. CFNIH3]
ATGAAACCTCAATCTGCAACAACTTCGCGGGCGGCGAAGCCTTGTCTGTCGCCCGTTGCAGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGAGCTGGTTCGAATAGATCGCAAGGGGTTT
AGCTGGCAGGAACAATCACCTTATCTTCGGGCCTTTGATATTCTGCGAGCGCGACATGCAACGGGTCTGTTACGACACAG
TCGTAACAACACGGCAATCTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11911.64 Da        Isoelectric Point: 6.9463

>AT93727 WP_064547036.1 NZ_CP026235:545561-545878 [Citrobacter freundii complex sp. CFNIH3]
MSNITWGLRRDITPRLGARLVQEGNRLHYLADRASMTGKFSDAECQKLDEIFPHFISQMESMLTTGELNPLHVHCVTLYH
TGFTCEADTLGSCGYVYIAIYPTKR

Download         Length: 318 bp

>AT93727 NZ_CP026235:545561-545878 [Citrobacter freundii complex sp. CFNIH3]
ATGAGCAACATCACATGGGGCCTGCGGCGGGATATCACGCCACGTCTGGGAGCCCGTCTGGTGCAGGAGGGGAACCGGCT
GCATTATCTGGCTGACCGGGCCAGCATGACTGGCAAGTTCAGTGACGCCGAATGCCAGAAGCTGGATGAAATATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGACCACCGGTGAACTGAATCCCCTACACGTTCATTGCGTCACCCTGTACCAC
ACCGGTTTTACCTGCGAAGCCGACACCCTCGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTAAGCGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8S1Q2

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JQF2

References