93717

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 18712..19231 Replicon plasmid pKPC-ac48
Accession NZ_CP026219
Organism Aeromonas sp. ASNIH4

Toxin (Protein)


Gene name relE Uniprot ID A0A2L0U0N7
Locus tag C2U40_RS24840 Protein ID WP_068979439.1
Coordinates 18944..19231 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A2L0U081
Locus tag C2U40_RS24835 Protein ID WP_068979438.1
Coordinates 18712..18954 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U40_RS24805 13827..14204 + 378 Protein_18 DUF4158 domain-containing protein -
C2U40_RS24810 14218..15492 - 1275 WP_103828292.1 IS4-like element ISApu2 family transposase -
C2U40_RS24815 15649..16020 + 372 WP_004099020.1 hypothetical protein -
C2U40_RS24820 16338..17606 + 1269 WP_011191342.1 IS4-like element ISApu1 family transposase -
C2U40_RS24825 17674..17907 - 234 WP_068979436.1 hypothetical protein -
C2U40_RS24830 17904..18533 - 630 WP_068979437.1 AAA family ATPase -
C2U40_RS24835 18712..18954 + 243 WP_068979438.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
C2U40_RS24840 18944..19231 + 288 WP_068979439.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C2U40_RS24845 19414..20412 + 999 WP_068979440.1 replication initiation protein -
C2U40_RS24850 20567..20857 - 291 WP_042650710.1 hypothetical protein -
C2U40_RS24855 21066..21479 + 414 WP_043849774.1 plasmid mobilization relaxosome protein MobC -
C2U40_RS24860 21469..23085 + 1617 WP_082044045.1 relaxase/mobilization nuclease domain-containing protein -
C2U40_RS24865 23123..23410 + 288 WP_043849770.1 hypothetical protein -
C2U40_RS24875 23791..24078 + 288 WP_043849769.1 CopG family ribbon-helix-helix protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 - 1..35298 35298


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(2-62)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11370.15 Da        Isoelectric Point: 9.7908

>T93717 WP_068979439.1 NZ_CP026219:18944-19231 [Aeromonas sp. ASNIH4]
MTFELEFDPRAWKEWKKLGDTVRQQFKKKLSEVIERPRIEANRLNGMSDCYKIKLRGAGYRLVYQVQDDRVVVFVVAVGR
REREEVYLDAMSRLD

Download         Length: 288 bp

>T93717 NZ_CP026219:18944-19231 [Aeromonas sp. ASNIH4]
ATGACCTTTGAACTCGAGTTTGATCCGCGAGCGTGGAAAGAGTGGAAAAAGTTGGGCGATACCGTTCGCCAGCAGTTCAA
AAAGAAACTTTCAGAGGTGATCGAGCGCCCCAGGATTGAGGCAAACAGGCTGAATGGGATGTCTGATTGCTACAAGATCA
AATTGCGTGGCGCGGGGTATCGGTTGGTTTACCAGGTACAGGATGATCGGGTGGTGGTATTCGTGGTCGCAGTGGGGAGG
CGCGAACGGGAAGAGGTATATCTGGATGCCATGTCACGTCTTGATTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8871.16 Da        Isoelectric Point: 4.7571

>AT93717 WP_068979438.1 NZ_CP026219:18712-18954 [Aeromonas sp. ASNIH4]
MGTINLRIDDELKARSFAVLDKLGVTPSDIIRQTLEYVASQGRSPFRTTVVTEDEEALLAIVRERLASPQKGIKVSLDDL

Download         Length: 243 bp

>AT93717 NZ_CP026219:18712-18954 [Aeromonas sp. ASNIH4]
ATGGGCACGATTAACCTGCGCATTGATGATGAACTGAAGGCACGTTCATTTGCGGTGCTGGACAAATTGGGGGTGACCCC
CTCGGATATTATCCGGCAAACCCTGGAGTATGTGGCCTCTCAGGGGCGGAGCCCGTTTCGCACTACCGTGGTGACGGAGG
ACGAAGAGGCACTGTTAGCCATCGTGCGAGAGCGCTTGGCCAGCCCGCAGAAAGGCATCAAGGTATCGCTGGATGACCTT
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L0U0N7

Antitoxin

Source ID Structure
AlphaFold DB A0A2L0U081

References