Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 4459609..4460199 Replicon chromosome
Accession NZ_CP026216
Organism Citrobacter sp. CFNIH10

Toxin (Protein)


Gene name pemK Uniprot ID A0A3S5G4X7
Locus tag C2U53_RS25830 Protein ID WP_039591092.1
Coordinates 4459609..4459941 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag C2U53_RS25835 Protein ID WP_000288812.1
Coordinates 4459942..4460199 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U53_RS25815 4455598..4456860 + 1263 WP_043000263.1 integrase arm-type DNA-binding domain-containing protein -
C2U53_RS25820 4457891..4458775 - 885 WP_104446677.1 integrase domain-containing protein -
C2U53_RS25830 4459609..4459941 - 333 WP_039591092.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
C2U53_RS25835 4459942..4460199 - 258 WP_000288812.1 antitoxin Antitoxin
C2U53_RS25845 4460547..4460753 - 207 WP_023243732.1 helix-turn-helix domain-containing protein -
C2U53_RS25850 4460750..4461223 - 474 WP_104446678.1 hypothetical protein -
C2U53_RS25855 4461582..4463537 + 1956 Protein_4260 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11765.62 Da        Isoelectric Point: 10.5834

>T93712 WP_039591092.1 NZ_CP026216:c4459941-4459609 [Citrobacter sp. CFNIH10]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLEGAGTKTTGVIRCDQPRTI
DMGARNGKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T93712 NZ_CP026216:c4459941-4459609 [Citrobacter sp. CFNIH10]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCAGGTCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCCGTTGTTGTTCCCGTCACCAGCGGTGGAAACTTCGCCCGCG
CAGCCGGTTTTACCGTCTCACTGGAGGGGGCGGGAACAAAAACAACGGGCGTGATCCGCTGCGACCAGCCCAGAACTATT
GATATGGGCGCCCGAAACGGTAAGCGACTGGAGCGCATACCCGACGCTGTCGTGAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTGAGCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT93712 WP_000288812.1 NZ_CP026216:c4460199-4459942 [Citrobacter sp. CFNIH10]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT93712 NZ_CP026216:c4460199-4459942 [Citrobacter sp. CFNIH10]
ATGTATACCACTCGTCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGCTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGTGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5G4X7

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References